Accuris Taq DNA Polymerase and Master Mix
Accuris Taq DNA Polymerase provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher processivity and greater template affinity, resulting in consistently superior performance. Accuris Taq Polymerase exhibits a 5 to 3 nuclease activity, but no 3 to 5 (proofreading) activity and works well with a wide range of DNA templates including GC-rich sequences.
· Ideal for routine PCR applications as well as genotyping, colony PCR and fast PCR.
· Improved template affinity and increased processivity for higher enzymatic activity and greater yields.
· Proprietary buffer optimized for a variety of assay conditions.
· Supplied with 5X buffer or as a convenient 2X Master Mix.
The polymerase is supplied with a 5X buffer containing MgCl2 and a proprietary mix of enhancers (dNTPs not included). For convenience, Accuris Taq is also available in a ready to use 2X Master Mix - just add primers and template DNA. The Master Mix can be ordered with a red loading dye that allows samples to be loaded directly on an agarose gel.
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请规范发贴，求助贴请在标题前加【求助】，谢谢您的合作。——by草根
DNA拓扑异构酶Ⅱ与抗肿瘤药物靶点的研究进展.doc(59.5k)
B、酶细胞产能细胞内（外）起作用消化酶细胞外起作用B错误；
C、酶同需要适条件同胃蛋白酶与胰蛋白酶所需PH同C错误；
D、葡萄糖细胞质基质解丙酮酸需要酶催化D确．
故选：D．
⒈通过嵌入DNA双链的碱基之间，形成稳定复合物，抑制DNA复制与RNA合成，从而阻碍快速生长的癌细胞的分裂。
⒉抑制拓扑异构酶II，影响DNA超螺旋转化成为松弛状态，从而阻碍DNA复制与转录。有研究显示拓扑异构酶II抑制剂（除蒽环类药物还包括依托泊苷等）能够阻止拓扑异构酶II的翻转，而这点对于它从它的核酸底物上脱离是必需的。这就意味着，拓扑异构酶II抑制剂使拓扑异构酶II的复合物在DNA链断裂之后才能更稳定，导致后者催化了DNA的破坏；同时，拓扑异构酶II抑制剂还能阻碍连接酶对DNA的修复。
⒊螯合铁离子后产生自由基从而破坏DNA、蛋白质及细胞膜结构。向左转|向右转
最近遇到了实验上的大问题，就是用一步法做RNA的荧光定量PCR时，发现低浓度的做不出来，而DNA是可以的，想请问各位在一步法PCR中有没有什么高见，就是逆转录的Buffer有没有什么需要注意的地方！就是在逆转录酶的最适Buffer和Taq酶的最适Buffer中寻找一个最适的Buffer！
ResponseofskeletalmuscleRNApolymerasesIandIItotumourgrowth
BiochimicaetBiophysicaActa(BBA)-GeneStructureandExpression,Volume950,Issue3,7September1988,Pages296-302
GeorgeA.J.Goodlad,CatherineM.Clark
或者有高手知道怎么测也行
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