Product information | Key steps | References | Technical support | Reviews |
Product overview
Product name | Normetanephrine ELISA kit - Plasma - Fast-Track |
Description | In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of free normetanephrine (normetadrenaline) in plasma samples. |
Labels | IVD, CE |
Format | 96-well plate |
Samples | plasma |
Sample volume | 500 µL |
Cross specie-reactivity | React with all species |
Standard range | 0 / 48 - 4 800 pg/ml |
Sensitivity | 23 pg/ml |
Specificity | No significant cross-reactivity was observed with Normetanephrine analogs such as Metanephrine, 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, VMS, HVMS, L-Dopa, L-Tyrosine, Tyramine and Acetaminophen. |
Assay time | Sample preparation and acetylation 30 min and ELISA overnight |
Storage | Store at 2-8°C for to 6 months. |
Datasheets | Instructions for use , Material safety datasheet |
Key steps
Sample collection & storage | EDTA- or citrate-plasma sample only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C. |
Sample preparation | Sample precipitation and acetylation (30min) |
ELISA | Normetanephrine antiserum overnight incubation, revelation and read steps (1h). |
Detailed protocol | Download instructions for use |
References
- Manz B et al. Development of enantioselective immunoassays for free plasma metanephrines. Ann N Y Acad Sci. 2004 Jun;1018:582-7.
- Gao YC et al. Comparison of free plasma metanephrines enzyme immunoassay with (131)I-MIBG scan in diagnosis of pheochromocytoma. Clin Exp Med. 2008 Jun;8(2):87-91.
- Procopiou M et al. Evaluation of an enzyme immunoassay for plasma-free metanephrines in the diagnosis of catecholamine-secreting tumors. Eur J Endocrinol. 2009 Jul;161(1):131-40.
- Christensen TT et al. Comparison of plasma metanephrines measured by a commercial immunoassay and urinary catecholamines in the diagnosis of pheochromocytoma. Scand J Clin Lab Invest. 2011 Dec;71(8):695-700.
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如题~
已经基本养好了贴壁的原代细胞了,准备鉴定了,看到好多都在说细胞爬片的问题,我可以不做爬片直接在皿里操作吗?具体需要注意些什么呢?感谢各位大神
所谓上皿式指的是称量盘在支架上面;而称量盘吊挂在支架下面的为下皿式。
目前使用较为广泛的是上皿式电子天平。
它最初由在德国生物学家罗伯特·科赫手下工作的细菌学家朱利斯·理查德·佩特里(1852-1921)于1887年设计,故又称为“佩特里皿”。
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其实就是常见的圆形的上下盖培养皿,你可以看看它的英文,就叫做petri dish,直译过来就叫做——佩特里小碟子——也就是皮氏培养皿
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第二,三段为原创手打……第一段为摘抄
激光共聚焦的看活细胞皿哪里买呢
各位战友,小弟刚开始养细胞,但是细胞状态一直不好,最近一次传代后,一传二,居然两个皿里细胞状态完全不同,第一个皿还能看见细胞的形态,而第二个皿几乎看不见贴壁的细胞,请问大家遇见过这种情况吗?
实验需要请问有没有养THP-1细胞的同仁?能否寄一皿我,我在广州南方医科大学,不胜感激!
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