Hito CryoMyelinStain™ Plus Kit (Gold phosphate complex Myelin Staining Kit with Hematoxylin Counter Stain) is made in a ready-to-use format and offers high quality, rapid staining of myelin/myelinated axons and nuclear counterstaining on frozen sections (mounted or floating). This kit has many advantages compared to the traditional Luxol fast blue staining method which is time-consuming, requires usage of 40-56°C incubator, and usually has low yields of stained myelin fibers and unreliable results. In addition, the long time high temperature incubation process of the frozen sections may cause sections felling off from the slides. Hito CryoMyelinStain™ Plus Kit offers a simple solution to these problems. The procedures are simplified and the processing time is greatly reduced. Users can use mounted sections or floating sections at room temperature. This kit delivers stable and improved staining quality. It has been proven to be extremely reliable and sensitive for demonstrating the morphological details of myelin fibers.
Myelin is essential for the proper functioning of the nervous system. Demyelination impairs the conduction of signals in the affected nerves, causing impairment in sensation, movement, and cognition. Currently no cure exists for demyelinating diseases and myelin repair is an active research field. Hito Hito CryoMyelinStain™ Plus Kit allows sensitive localization and visualization of the myelin fibers, thus offers a fast and reliable way to determine the extent of demyelination.
Hito CryoMyelinStain™ Plus Kit has been tested extensively on the brains and spinal cords from several species of animals and it is a simple solution for your research.
| Kit Contents (for 60 slides or 200 sections) | |
| Solution-1 | 50 ml |
| Solution-2 | 3 ml |
| Solution-3 | 12 ml |
| Solution-4 | 250 ml |
| Solution-5 | 25 ml |
| Solution-6 | 30 ml |
| Solution-7 | 125 ml |
| Staining Jar | 3 |
| Hito Aqua Barrier PAP Pen (HTHS0110) | 1 |
| Fine Tip Natural Hair Brush | 1 |
| Glass Specimen Transfer Tool | 1 |
| User Manual and MSDS | 1 |
Before using Hito CryoMyelinStain Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Cryostat and light microscopeDry ice, isopentane, O.C.T. compound, ethanol, xylene, 4% PFA (recommend Hito Buffered 4% Paraformaldehyde Solution Cat# HTSHS0102), double distilled or deionized waterGelatin coated slides and coverslipsStaining jars for slides washResinous mounting medium  Hito CryoMyelinStain™ Plus Kit Manual and MSDS | |
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人教版高中生物必修二有这个图
1、测浓度和纯度，是否达标
2、跑电泳看条带是否与测的数据相互印证
如果上述两点满足，就不需要纯化，否则需要进一步纯化。
不过根据经验，一般都不需要，我们实验室用的是GNT系列的试剂盒，提取结果就直接进入下游实验了，效果挺稳定
质粒是真核细胞细胞核外或原核生物拟核区外能够进行自主复制的遗传单位,包括真核生物的细胞器（主要指线粒体和叶绿体）中和细菌细胞拟核区以外的环状脱氧核糖核酸(DNA)分子.现在习惯上用来专指细菌(大肠杆菌）、酵母菌和放线菌等生物中细胞核或拟核中的DNA以外的DNA分子.
小鼠没有叶绿体,你可以直接参照小鼠线粒体的提取方法进行提取.
想搞清一个问题，如何区别是因为small RNA 引起的调控呢？还是tRNA引起的调控，有没有有效的分离这两种RNA的办法呢？
暂无品牌问答
