Hito CryoMyelinStain™ Plus Kit (Gold phosphate complex Myelin Staining Kit with Hematoxylin Counter Stain) is made in a ready-to-use format and offers high quality, rapid staining of myelin/myelinated axons and nuclear counterstaining on frozen sections (mounted or floating). This kit has many advantages compared to the traditional Luxol fast blue staining method which is time-consuming, requires usage of 40-56°C incubator, and usually has low yields of stained myelin fibers and unreliable results. In addition, the long time high temperature incubation process of the frozen sections may cause sections felling off from the slides. Hito CryoMyelinStain™ Plus Kit offers a simple solution to these problems. The procedures are simplified and the processing time is greatly reduced. Users can use mounted sections or floating sections at room temperature. This kit delivers stable and improved staining quality. It has been proven to be extremely reliable and sensitive for demonstrating the morphological details of myelin fibers.
Myelin is essential for the proper functioning of the nervous system. Demyelination impairs the conduction of signals in the affected nerves, causing impairment in sensation, movement, and cognition. Currently no cure exists for demyelinating diseases and myelin repair is an active research field. Hito Hito CryoMyelinStain™ Plus Kit allows sensitive localization and visualization of the myelin fibers, thus offers a fast and reliable way to determine the extent of demyelination.
Hito CryoMyelinStain™ Plus Kit has been tested extensively on the brains and spinal cords from several species of animals and it is a simple solution for your research.
| Kit Contents (for 60 slides or 200 sections) | |
| Solution-1 | 50 ml |
| Solution-2 | 3 ml |
| Solution-3 | 12 ml |
| Solution-4 | 250 ml |
| Solution-5 | 25 ml |
| Solution-6 | 30 ml |
| Solution-7 | 125 ml |
| Staining Jar | 3 |
| Hito Aqua Barrier PAP Pen (HTHS0110) | 1 |
| Fine Tip Natural Hair Brush | 1 |
| Glass Specimen Transfer Tool | 1 |
| User Manual and MSDS | 1 |
Before using Hito CryoMyelinStain Kit, please make sure you have the following Required Equipment / Materials in your lab (not included in the kit):Cryostat and light microscopeDry ice, isopentane, O.C.T. compound, ethanol, xylene, 4% PFA (recommend Hito Buffered 4% Paraformaldehyde Solution Cat# HTSHS0102), double distilled or deionized waterGelatin coated slides and coverslipsStaining jars for slides washResinous mounting medium  Hito CryoMyelinStain™ Plus Kit Manual and MSDS | |
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提RNA一般注意：提取前材料的保存（新鲜材料,过夜处理的样品）,提取中对外源性的RNA酶的清除控制（离心管,PCR管,电泳槽,台面等的无RNA酶化处理）,提取后需要-80度保存.其次跑电泳时,注意电泳液最好用新鲜的,核酸染料等诸多因素.
细菌总RNA提取试剂盒（ germs islation kit).
对于外源性RNA酶的控制外源RNA酶清除剂,避免使用致癌物DEPC的危险方法就可简单操作清除耗材,台面,仪器等的外源RNA酶的降解作用.
我在一些文献上看到说，要加入tRNA as carrier，但是这句话不好理解
有没有人用过，跟我讲讲tRNA到底有什么用
多谢指教
1、测浓度和纯度，是否达标
2、跑电泳看条带是否与测的数据相互印证
如果上述两点满足，就不需要纯化，否则需要进一步纯化。
不过根据经验，一般都不需要，我们实验室用的是GNT系列的试剂盒，提取结果就直接进入下游实验了，效果挺稳定
质粒是真核细胞细胞核外或原核生物拟核区外能够进行自主复制的遗传单位,包括真核生物的细胞器（主要指线粒体和叶绿体）中和细菌细胞拟核区以外的环状脱氧核糖核酸(DNA)分子.现在习惯上用来专指细菌(大肠杆菌）、酵母菌和放线菌等生物中细胞核或拟核中的DNA以外的DNA分子.
小鼠没有叶绿体,你可以直接参照小鼠线粒体的提取方法进行提取.
1、测浓度和纯度，是否达标
2、跑电泳看条带是否与测的数据相互印证
如果上述两点满足，就不需要纯化，否则需要进一步纯化。
不过根据经验，一般都不需要，我们实验室用的是GNT系列的试剂盒，提取结果就直接进入下游实验了，效果挺稳定
质粒是真核细胞细胞核外或原核生物拟核区外能够进行自主复制的遗传单位,包括真核生物的细胞器（主要指线粒体和叶绿体）中和细菌细胞拟核区以外的环状脱氧核糖核酸(DNA)分子.现在习惯上用来专指细菌(大肠杆菌）、酵母菌和放线菌等生物中细胞核或拟核中的DNA以外的DNA分子.
小鼠没有叶绿体,你可以直接参照小鼠线粒体的提取方法进行提取.
实验非常不顺，想构建CDNA文库，但是从mRNA开始屡次失败，考虑主要是纯化过程中损失过多。想从总RNA入手，但是不知道实验步骤。不知那位大侠能提供总RNA建库的实验步骤。另外我现在手头有OligoDT,RT酶，苦于没有第二连合成试剂盒，不知道能否用普通PCR试剂盒Teq酶替代第二链合成过程中的DNA聚合酶I。好像有种方法合成第二链时，不需要另外的引物，利用降解的RNA作引物即可，我想直接设定PCR两个循环，合成第二链，不知方法可行否？愁啊，等着毕业，时间紧急，恳请帮忙。谢谢。
mRNA 数量不详，根据转录数量，加工数量各不相同，故无法得知。
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