
Biotin-Linked Monoclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB)
VEGF-B; VEGFL; VRF; VEGF-Related Factor
- Product No.LAA144Hu72
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- SourceAntibody labeling
- Ig IsotypeIgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelBiotin
- Original Antibodyn/a
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC.If the antibody is used in flow cytometry, please check FCM antibodies.
- Downloadn/a
- UOM20µl100µl200µl1ml10ml
- FOBUS$ 111 For more details, please contact local distributors!US$ 258 For more details, please contact local distributors!US$ 369 For more details, please contact local distributors!US$ 923 For more details, please contact local distributors!US$ 3690 For more details, please contact local distributors!
SPECIFITY of the Biotin-Linked Monoclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB)
The antibody isa mouse monoclonal antibody raised against VEGFB. It has been selected for its ability to recognize VEGFB in immunohistochemical staining and western blotting.
USAGE of the Biotin-Linked Monoclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB)
Western blotting: 0.2-2µg/mL;1:500-5000 Immunohistochemistry: 5-20µg/mL;1:50-200 Immunocytochemistry: 5-20µg/mL;1:50-200 Optimal working dilutions must be determined by end user.
STORAGE of the of the Biotin-Linked Monoclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Biotin-Linked Monoclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Protein A/G Purification ColumnStaining Solution for Cells and TissuePositive Controlfor AntibodyTissue/Sections Customized ServicePhosphorylated Antibody Customized ServiceWestern Blot (WB) Experiment ServiceImmunohistochemistry (IHC) Experiment ServiceImmunocytochemistry (ICC) Experiment ServiceFlow Cytometry (FCM) Experiment ServiceImmunoprecipitation (IP) Experiment ServiceImmunofluorescence (IF) Experiment ServiceBufferDAB Chromogen KitSABC KitReal Time PCR Experimental Service
Related products
Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
RPA144Hu01 | Recombinant Vascular Endothelial Growth Factor B (VEGFB) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA144Hu01 | Active Vascular Endothelial Growth Factor B (VEGFB) | Cell culture; Activity Assays. |
PAA144Hu01 | Polyclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB) | WB; IHC; ICC; IP. |
LAA144Hu71 | Biotin-Linked Polyclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB) | WB; IHC; ICC. |
LAA144Hu81 | FITC-Linked Polyclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB) | WB; IHC; ICC; IF. |
MAA144Hu22 | Monoclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB) | WB; IHC; ICC; IP. |
LAA144Hu82 | FITC-Linked Monoclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB) | WB; IHC; ICC; IF. |
LAA144Hu72 | Biotin-Linked Monoclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB) | WB; IHC; ICC. |
SEA144Hu | ELISA Kit for Vascular Endothelial Growth Factor B (VEGFB) | Enzyme-linked immunosorbent assay for Antigen Detection. |
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有哪位比较明白的能给我提点建议么,这两天真是晕到家了。谢谢!
请问一下做流式需要的抗体那种比较好,有人说Biolegend,eBioscience,santa,BD。求各位大神指教
anti-mouse TCR γ/δ Antibody
anti-human TCR γ/δ Antibody
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
多抗虽然可能可以产生更强的信号,但是特异性差,染色后荧光强度和抗原水平不是线性关系,不同批次差异很大。所以流式检测不使用多抗。
比如,你使用了小鼠IgG1, PE 标记的特异性抗体,那就选用小鼠IgG1, PE标记的非特异性抗体。
下面这个图,是一个比较典型的使用了同型对照的结果图向左转|向右转灰色的曲线是未染色的样本,黄色是同型对照,红色是特异性抗体染色。由于细胞对抗体的非特异性结合,一般同型对照都会比未染色样本的荧光信号要强。所以设置阴性阈值的时候,要根据同型对照,而不是未染色的细胞。


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