Product Description
Product Name | Cat# | Clone# | Isotype | Applications | Reactivity | Unit/Size |
Acetyl-alpha tubulin (Lys40) Monoclonal Antibody | E-AB-20302 | 6B3 | IgG | WB,IHC-p,IF* | Human,Mouse,Rat | 20μL, 60μL, 120μL, 200μL |
Description
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Spec Sheet E-AB-20302 (PDF File)
Synonyms | Anti-Acetyl-alpha tubulin (Lys40) |
Swiss-Prot | P68363 |
Concentration | 1mg/mL |
Host | Mouse |
Immunogen | Synthetic Peptide |
Purification Method | Protein A purification |
Formulation | PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4 |
Storage | Store at -20?. Avoid freeze / thaw cycles. |
Dilution | WB 1:1000-2000, IHC 1:50-100, IF 1:200 |
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. The vial is prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
* Remark Icon :
WB=Western Blotting, IP=Immunoprecipitation, IF=Immunofluorescence, IHC=Immunohistochemistory, IHC-p=Immunohistochemistory Paraffin, FCM=Flow Cytometry, CH=ChIP Assay
Manufactured by:Elabscience
ebiomall.com
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我用简并引物从鸡cDNA中扩增出一个片段,约250bp,测序后拿来BLAST时却发现,与之同源性最高的并不是其它的Mx基因,而是人的某一个基因,比对的结果中与该序列同源性较高的有人及鼠Mx基因,但是同源性并不是很高。该基因在同一种生物的不同品种中具有多态性,已经有其它的鸡的Mx基因被克隆,按道理如果该片段是Mx基因的一部分它应该与其它的鸡的Mx基因有很高的同源性才对呀,所以,我怀疑简并引物PCR扩增出来的该片段不是Mx基因,因为RACE试剂盒很贵,现在也不敢继续作下去,怕作下去得不到什么结果。现在贴上我BLAST结果中score排在最前面的部分,还请有经验的兄弟帮我分析分析。我不胜感激。
Sequencesproducingsignificantalignments:(bits)Value
gi|19807872|gb|AC087694.3|Homosapienschromosome8,clone...460.033
gi|19073805|gb|AC090733.7|Homosapienschromosome8,clone...460.033
gi|38081550|ref|XM_358891.1|MusmusculussimilartoMyxovi...440.13
gi|47026513|gb|AC024957.9|Musmusculuschromosome16,clon...440.13
gi|13435314|gb|AC090999.1|CaenorhaBDitiseleganscosmidY8...440.13
gi|21955645|emb|AL732599.5|MouseDNAsequencefromcloneR...440.13
gi|199921|gb|J03368.1|MUSMX2Mouseinterferon-inducedMx2m...440.13
gi|31442540|gb|AC138314.4|MusmusculusBACcloneRP23-218B...420.52
gi|21591808|gb|AC092491.6|Homosapiens12BACRP11-125G9(...420.52
gi|40949625|gb|AC127597.5|MusmusculusBACcloneRP23-71A1...420.52
gi|6996929|ref|NM_010846.1|Musmusculusmyxovirus(influen...402.1
gi|15029783|gb|BC011113.1|Musmusculusmyxovirus(influenz...402.1
gi|13938021|gb|BC007127.1|Musmusculusmyxovirus(influenz...402.1
gi|19705454|ref|NM_134350.1|Rattusnorvegicusmyxovirus(i...402.1
gi|16041423|gb|AC091589.8|Homosapienschromosome18,clon...402.1
gi|47104230|gb|BT012815.1|Lycopersiconesculentumclone11...402.1
gi|20270172|gb|AC114485.2|Homosapienschromosome1clone...402.1
gi|28372591|gb|AC008939.8|Homosapienschromosome5clone...402.1
gi|19310326|gb|AC105250.3|HomosapiensBACcloneRP11-39C1...402.1
gi|37537322|dbj|BS000055.1|Pantroglodyteschromosome22c...402.1
gi|37537321|dbj|BS000054.1|Pantroglodyteschromosome22c...402.1
gi|18464281|gb|AC104690.2|HomosapiensBACcloneRP11-183P...402.1
gi|15321560|gb|AC079232.7|HomosapiensBACcloneRP11-360H...402.1
gi|56724|emb|X52713.1|RNMX3RatmRNAforMx3protein402.1
gi|56722|emb|X52712.1|RNMX2RatmRNAforMx2protein402.1
gi|56720|emb|X52711.1|RNMX1RatmRNAforMx1protein402.1
我的信箱:wjy_0912@hotmail.com
是不是只要从细胞里面提取基因组DNA,然后按照pubmed上的5端上游序列做一个PCR就可以了?
用引物PCR扩增目基片段;
选择合适(抗性标记、酶切位点等)克隆载体(保真扩增),并PCR片段连接入克隆载体;(般用Taq酶PCR产物末尾自带A,Solution 1作用与两端各带T线性T载体直接相连)
连接产物转化入受态肠杆菌,使含抗素培养基扩增;
肠杆菌提取质粒(即前面连接产物),酶切鉴定测序鉴定均误目基片段切并与新表达载体连接,再转化入肠杆菌扩增,再提质粒,即想要目基片段克隆.
目基表达基工程叙述~
DNA杂交技术
DNA-RNA杂交技术
抗体抗原杂交技术
我现在做的课题研究的是肾脏肿瘤。其中有实验涉及到FISH。咨询和查阅过相关文献和公司,一个染色体荧光探针的价格贵的惊人(动不动近万),做过这方面实验的老师,情况是这样的吗?
我要做的是在石蜡切片上进行FISH实验,操作难度是不是很大?有没有公司可以帮助完成此类实验呢?
请知道的老师给我帮助,谢谢!!
大家好,我最近在克隆一个基因的全长序列,用的是cDS,之前的引物在NCBI上Blast,是特异引物,一开始没有条带,通过不断的试程序,重提RNA等,出现条带了,大小也对,结果测序不是我的基因,然后重新换引物,现在的情况是,51度有我的条带,但是有很多杂带,升高退火温度后,以及减少循环后,啥也没有了,求大神指点。
暂无品牌问答