The Yeast Processing Reagent (for total RNA preparation) is a pretreatment reagent that may be used in combination with RNApurificationreagents toenable RNA isolation from yeast. Pretreatment of yeast cells with this product comprises two steps: 1) washing and recovery of yeast cells by centrifugation, and 2) yeast cell wall disruption via a Yeast Processing Enzyme Solution (containing a cell wall degradation enzyme) and a Yeast Processing Buffer.
The Yeast Processing Reagent (for total RNA preparation) is a pretreatment reagent that may be used in combination with RNApurificationreagents toenable RNA isolation from yeast. Pretreatment of yeast cells with this product comprises two steps: 1) washing and recovery of yeast cells by centrifugation, and 2) yeast cell wall disruption via a Yeast Processing Enzyme Solution (containing a cell wall degradation enzyme) and a Yeast Processing Buffer. Becauseyeast cell walldisruption occurs throughenzymatic activity, the time-consuming cell-lysis protocols using glass beads and liquid nitrogen required in conventional methods are unnecessary.
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takara的做得出来不,请各位高手指教,谢谢了!
求采纳
Clontech的非常好, 感觉. 效果不好可能是RNA问题, 或RTase的问题. 因为目前clontech的RTase是Takara的产品,而非原来clontech的产品,可单购。
请采纳。。。。
3′-Full RACE Core Set Ver.2.0D314 20 次 1,800 元
5′-Full RACE Kit D315 RT反应10次 PCR反应50 次 3,980 元
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