Description
The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX) is designed for reverse transcription and quantitative real-time analysis of a specific target RNA by one-step reaction. The ExcelRT™ One-Step RT-qPCR kit (TaqMan, ROX), consisting of One-Step RT Enzyme Mix and 2X One-Step qPCR Master Mix, is a convenient kit designed for highly efficient cDNA synthesis and highly specific real-time PCR in a single tube. The One-Step RT Enzyme Mix contains a thermostable ExcelRT™ Reverse Transcriptase and a RNAok™ RNase inhibitor. Consequently, One-Step RT Enzyme Mix can reverse transcribe RNA to cDNA at a wide temperature range from 42 to 60°C and active against RNase A, RNase B and RNase C. By containing specialized hot-start Taq DNA polymerase, which greatly reduce primer-dimer formation and can be activated within 2 minutes, the 2X One-Step qPCR Master Mix features high specificity and is suitable for fast cycle program. This master mix includes ROX reference dye for normalization of each RT-qPCR assay.
Features
High yield
Reverse transcription at wide temperature range (42-60°C)
High specificity
Suitable for fast cycle program
With ROX reference dye
Storage
Aliquot to avoid multiple freeze-thaw cycles (stable within 30 freeze-thaw cycles)
Protect from light
-20°C for 12 months
Reverse transcription at wide template range
ExcelRT™ One-Step RT-qPCR Kit can quantitative analyze target RNA from a wide range of RNA template input. The amplification plot of one-step RT-qPCR with total RNA templates ranging from 1 pg to 1 ng in quantity, analyzed by using RQ2110 ExcelRT™ One-Step RT-qPCR Kit (TaqMan, ROX) for RT-qPCR amplification.
Reverse transcription at wide temperature range (42-60°C)
ExcelRT™ One-Step RT-qPCR Kit can quantitative analyze target RNA at a wide temperature range (42-60°C). The amplification plot of one-step RT-qPCR with reverse transcription at temperature range from 42 to 60°C, analyzed by using RQ2110 ExcelRT™ One-Step RT-qPCR Kit (A) or kit from brand N (B) for RT-qPCR amplification. The overlapped amplification curves display that ExcelRT™ One-Step RT-qPCR Kit preforms successfully cDNA synthesis at wide temperature range.
Contents
Component | Volume |
One-Step RT Enzyme Mix | 400 μl |
2X One-Step qPCR Master Mix | 2 x1 ml |
Storage
Aliquot to avoid multiple freeze-thaw cycles (stable within 30 freeze-thaw cycles)
Protect from light
-20°for 12 months
Manual
Manual_RQ2110_ExcelRT™ 2X One-Step RT-qPCR Kit (TaqMan, ROX)
SDS
SDS_RQ2110
Recommended primer design
Amplicon size: 80-150 bp
Tm value: around 60°C (calculated with Primer3 software)
Primer length: 17-25 mer
Sequence:
‒ 45-55% of GC content isrecommended.
‒ Avoid regional high GC or ATcontent
‒ Avoid palindrome sequence
‒ Sequence with G or C at the 3" end is recommended.
Specificityof primers should be confirmed through a BLAST search.
Recommended probe design
Tm value: 6-10°C higher than primers
Probe length: 20-30 mer
Sequence:
‒ 35-65% of GC content is recommended.
‒ Avoid regional high GC or AT content
‒ Select the strand contains more C’s than G’s
‒ Avoid palindrome sequence
‒ Avoid a G at the 5" end to prevent quenching of the 5’fluorophore.
Specificityof probe should be confirmed through a BLAST search.
Recommended reaction mixture set up for qPCR
Component | Volume | Final concentration |
Template RNA | Varied | 1 pg – 1 μg |
Forward primer (10 μM) | Varied | 125 – 900 nM |
Reverse primer (10 μM) | Varied | 125 – 900 nM |
TaqMan Probe (10 μM) | Varied | 125 – 900 nM |
One-Step RT Enzyme Mix | 2 μl | 1X |
2X One-Step Master Mix | 10 μl | 1X |
ddH2O | to 20 μl | - |
Total volume | 20 µl | - |
*Template amount varies depending on the copy number of target present in the template solution.
** The PCR primer and probe concentration for an optimalqPCR reaction may vary according to primers’ and probe’s properties.
Recommended qPCRProgram
Standard program
Steps | Temp. | Time | Cycles |
Reverse transcription | 42°C - 60°C (45°C - 55°C is recommended) | 10min | 1 |
Enzyme activation | 95°C | 3 min | 1 |
Denaturation | 95°C** | 15 sec | 40-50 |
Annealing/ Extension | 60°C | 1 min |
Fast program
Steps | Temp. | Time | Cycles |
Reverse transcription | 42°C - 60°C (45°C - 55°C is recommended) | 5 min | 1 |
Enzyme activation | 95°C | 20 sec | 1 |
Denaturation | 95°C** | 3 sec | 40-50 |
Annealing/ Extension | 60°C | 30 sec |
[RP1000] ExcelRT™ Reverse Transcriptase
High yield
Thermostable, up to 50°C, during first strand synthesis
High processivity, generating cDNA up to 8 kb
Reduced RNase H ribonuclease activity
[RI1000] RNAok™ RNase Inhibitor
Application
RT-PCR
cDNA Synthesis
in vitro transcription
[RP1400] ExcelRT™ Reverse Transcription Kit II
Contains all components for reverse transcription
High yield
Thermostable, up to 50°C
Reduced RNase H ribonuclease activity
Suitable for real-time PCR
Contains all components for reverse transcription
High yield
Thermostable, up to 50°C
Reduced RNase H ribonuclease activity
Suitable for real-time PCR
[TQ2110] ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX)
High sensitivity
High specificity
With ROX reference dye
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
对于新手来说购买反转录试剂盒是比较理想的,买一个kit看看说明书操作就可以了。推荐两款kit TAKARA和HaiGene,这两款试剂盒都能对RNA样品中的gDNA有效去除,因此对RNA质量的要求不高。TAKARA的RR047A操作方便,反转录温度为37℃,对于大多数试验来讲是满足要求的。HaiGene的D0401操作要多一个步骤,但其反转录温度是55℃(耐高温的反转录酶),提高了反转录温度使得高GC含量、复杂模板、长mRNA的模板都能有效反转录,因此其反转录效率更高,更能够获得样本中基因的真实表达量。如果后续试验是RealTime PCR、ORF克隆、高GC含量、或者你的待研究基因结构复杂程度未知,还是选用耐高温的反转录酶更理想。对于反转录高手来说,直接购买反转录酶、再购买Rnase Inhibitor自己配制反转录体系就可以了。对于样本量大的课题组来讲,相对还是比较经济的。选择反转录酶时仅需要考虑是否需要耐高温的酶,来克服目的基因的复杂结构就可以了。PROMEGA、TAKARA、HaiGene、 TRANSGEN等品牌的反转录酶性价比还都是不错的。Life、NEB的也不错,不过性价比一般,不一一解释了。
2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBRGreenMix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBRGreen,并且如果你那是ABI或者Stratagene的PCR如果用SYBRGreen还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
大家miRNA逆转录和qPCR的试剂盒用的是那个公司的啊,求推荐!
转录的只是插入的片断,怎么会跟模板一样大呢,一般情况下应该比模板小多了。我都是跑电泳,严格来说应该跑变性的;嫌麻烦可以非变性,快速(防止RNA降解)十分钟即可;抛出来的带会有两条,上面大的是模板,下面一条比较亮的是RNA,那么你的转录就 成功了。
PCR第一个步骤如果没记错的话应该叫“变性”, PCR的话是不需要解旋酶的,直接利用DNA的高温变性特性,在高温下,使氢键断开,DNA双链恢复为单链。 (还有解旋酶好像是解除双螺旋的结构,是一种拓扑异构酶,对于氢键的打开没有贡献吧
加尾法是采用加A酶先对mirna进行加尾,然后再用带oligodt的引物反转录,他的反转录引物是通用的,一次反转录可以获得所有miRNA的cdna,效率高。茎环法是采用特异性反转录引物序列+颈环结构作为反转录引物进行反转录的,一次反转录只能获得一种mirna的cdna,可能有几种一起反转录的,这个我不太清楚一起反转录的效果。然后茎环法的经典即ABI的探针法定量试剂盒。我用过茎环法反转录+染料法定量的,下游引物为通用引物,结果不太好,就现在用的这家的他采用的是双向引物都是特异性的,效果还可以。不知道我说的清楚不,希望能对你有帮助。
如果是用转录出的cDNA做那跟平时就一样了。不需要特别的试剂盒、
1.模板提取(一般为RNA):Trizol、氯仿、异丙醇、无水乙醇、DEPC处理水
2.模板浓度测定:分光光度计或NanoDrop
3.逆转录:逆转录试剂盒(或者一步法试剂盒),这一步可以用普通PCR做,也可以用水域做。
4.荧光定量PCR试剂:通常有用SYBR Green Mix做的,但是这里建议你用EvaGreen做,灵敏度和平行性都要好于SYBR Green,并且如果你那是ABI或者Stratagene的PCR如果用SYBR Green还需要加一步Rox很麻烦。
5.其他:除了以上的那些还需要离心管、PCR管或板(Axygen反应比较好)、移液枪等,暂时就想到这么多。
暂无品牌问答