Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
---|---|---|---|---|---|---|
Plasmid | 83900 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
AAV Retrograde | 83900-AAVrg | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart |
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV(Search Vector Database)
- Vector typeAAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)DH5alpha
- Copy numberHigh Copy
Gene/Insert
- Gene/Insert nameeGFP
- PromotermDlx
Cloning Information
- Cloning methodGibson Cloning
- 5′ sequencing primerchim-int-F (5'-TCTTACTGACATCCACTTTGCC-3')
- 3′ sequencing primerWPRE-R (Common Sequencing Primers)
Resource Information
- Supplemental Documents
- Snapgene Full Map
- Terms and Licenses
- UBMTA
- Ancillary Agreement for Plasmids Containing FP Materials
- Industry Terms
- Not Available to Industry
- Articles Citing this Plasmid
- 3 References
Information for AAV Retrograde (Catalog # 83900-AAVrg)(Back to top)
Purpose
Ready-to-use AAV Retrograde particles produced from pAAV-mDlx-GFP-Fishell-1 (#83900). In addition to the viral particles, you will also receive purified pAAV-mDlx-GFP-Fishell-1 plasmid DNA.
mDlx-driven expression of GFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV retrograde (AAVrg)
- PurificationIodixanol gradient ultracentrifugation
- Reporter GeneGFP
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
Addgene Comments
Retrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
特点
零耐药性,零药物残留,调控瘤胃微生物,提高饲料吸收,提高产奶量、降低体细胞、防治隐性乳房炎。
主要成分
抗牛乳房炎特异性IgY、乳酸芽孢杆菌、嗜酸乳杆菌、螯合维生素、半胱胺肽、全卵蛋白肽、活性酵母菌、螯合铁、铜、锌,抗病因子、保健因子、有益菌、生物活性复合酶、生物活性小肽等多种高档原料。
产品性状
本品呈黄褐色粉末,有特别的发酵芳香味。
功能特点
采用“三步多相联合发酵”专利技术生产,经深层发酵,离心、真空低温干燥后,辅以免疫多糖和多种科学配制而成。
1、可彻底分解霉变饲料中的弱极性霉菌毒素,可完全替代预防用抗生素;
2、可明显提高奶牛生产的各个指标,试验证明提高产奶量8-20%。延长产奶高峰15-35天,提高乳脂率0.25-0.4%,同时提高乳蛋白含量0.02-0.05%;
3、对防治奶牛乳房炎有特效,可降低乳房炎发病率60-70%,同时防治奶牛酸奶症效果突出;
4、加快犊牛瘤胃发育,可有效降低腹泻率47%,提高饲料利用率2.5%;
5、生物学稳定性高,耐挤压、耐酸碱(PH3-9)、耐高温(90℃加热30分钟活菌损失不到1%);
6、减少粪便排泄量,可降低牛舍氨臭味,减少。
应用范围:
犊牛、青年牛、高产奶牛、干乳奶牛。
使用方法:
①犊牛本品1000g拌料1000-2000斤,如发生腹泻时本品1000g拌料500斤均匀混饲;
②青年牛和干乳奶牛本品1000g拌料1000-2000斤,均匀混饲;
③高产奶牛本品1000g拌料500-1000斤,均匀混饲,如遇天气突变、转群、噪音等各种应激情况本品1000g拌料500-1000斤添加使用。
望采纳!!
有没有知道哪个公司可以买到几种牛病毒的荧光标记的抗体?
我要做细胞的免疫荧光实验。
1.Bluetongue蓝舌病毒
2.BovineAdenovirus牛腺病毒
3.BovineParvovirus牛细小病毒
4.BovineRespiratorySyncytialVirus牛呼吸道融合病毒
5.BovineVirusDiarrhoea牛腹泻病毒
6.Reovirus呼肠孤病毒-3
7.InfectiousBovineRhinotracheitis,IBR传染性牛鼻气管炎病毒
8.BovineParainfluenzaVirusType3,PI3牛副流行性感冒3型病毒
9.RABIes狂犬病毒
能提供一种的也可以,多谢啊~~~~~~~
如题,求购做wb时适用于牛的抗纤连蛋白抗体,分装或者试用装皆可,不求多么高质量,做出来的东西能毕业就行,谢谢~~
暂无品牌问答