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Novus/Recombinant Human IL-4 Protein/50 ug/204-IL-050
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Novus/Recombinant Human IL-4 Protein/50 ug/204-IL-050
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Novus
货号 / 
204-IL-050
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RecombinantHumanIL-4ProteinSummary

AdditionalInformation
ANewrhIL-4isNowAvailable!Thenewproteinismammaliancellexpressed!
DetailsofFunctionality
MeasuredinacellproliferationassayusingTF‑1humanerythroleukemiccells.Kitamura,T.etal.(1989)J.CellPhysiol.140:323.TheED50forthiseffectis0.05-0.2ng/mL.ThespecificactivityofrecombinanthumanIL-4isapproximately2.9x104IU/μg,whichiscalibratedagainsthumanIL-4WHOInternationalStandard(NIBSCcode:88/656).
Source
E.coli-derivedhumanIL-4proteinHis25-Ser153,withanN-terminalMet
Accession#
P05112
N-terminalSequence
Met
Protein/PeptideType
RecombinantProteins
Gene
IL4
Purity
>97%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain.
EndotoxinNote
<0.10 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">

Applications/Dilutions

TheoreticalMW
15kDa.Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors.
SDS-PAGE
14kDa,reducingconditions
ReviewedApplications
Read2Reviewsrated5
using204-ILinthefollowingapplication:
  • CellProliferation
Publications
ReadPublicationsusing204-ILinthefollowingapplications:
  • Bioassay
    305publications
  • CellCulture
    9publications
  • ELISA(Standard)
    8publications
  • ExVivo
    1publication

Packaging,Storage&Formulations

Storage
Useamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
  • 12monthsfromdateofreceipt,-20to-70°Cassupplied.
  • 1month,2to8°Cundersterileconditionsafterreconstitution.
  • 3months,-20to-70°Cundersterileconditionsafterreconstitution.
Buffer
Lyophilizedfroma0.2μmfilteredsolutioninPBSwithBSAasacarrierprotein.
Purity
>97%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain.
ReconstitutionInstructions
Reconstituteat100-200μg/mLinsterilePBScontainingatleast0.1%humanorbovineserumalbumin.

Notes

ThisproductisproducedbyandshipsfromR&DSystems,Inc.,aBio-Technebrand.

AlternateNamesforRecombinantHumanIL-4Protein

  • Bcellgrowthfactor1
  • BCDF
  • B-cellstimulatoryfactor1
  • BCGF1
  • BCGF-1
  • binetrakin
  • BSF1
  • BSF-1
  • IL4
  • IL-4
  • IL-4B_cellstimulatoryfactor1
  • interleukin4
  • interleukin-4
  • Lymphocytestimulatoryfactor1
  • MGC79402
  • pitrakinra

Background

Interleukin-4(IL-4),alsoknownasBcell-stimulatoryfactor-1,isamonomeric,approximately13kDa‑18kDaTh2cytokinethatshowspleiotropiceffectsduringimmuneresponses(1‑3).Itisaglycosylatedpolypeptidethatcontainsthreeintrachaindisulfidebridgesandadoptsabundledfouralpha-helixstructure(4).HumanIL-4issynthesizedwitha24aasignalsequence.Alternatesplicinggeneratesanisoformwitha16aainternaldeletion.MaturehumanIL-4shares55%,39%and43%aasequenceidentitywithbovine,mouse,andratIL-4,respectively.Human,mouse,andratIL-4arespecies-specificintheiractivities(5‑7).IL-4exertsitseffectsthroughtworeceptorcomplexes(8,9).ThetypeIreceptor,whichisexpressedonhematopoieticcells,isaheterodimeroftheligandbindingIL-4Ralphaandthecommongammachain(asharedsubunitofthereceptorsforIL-2,-7,-9,-15,and‑21).ThetypeIIreceptoronnonhematopoieticcellsconsistsofIL-4RalphaandIL‑13Ralpha1.ThetypeIIreceptoralsotransducesIL-13mediatedsignals.IL-4isprimarilyexpressedbyTh2-biasedCD4+Tcells,mastcells,basophils,andeosinophils(1,2).Itpromotescellproliferation,survival,andimmunoglobulinclassswitchtoIgG4andIgEinhumanBcells,acquisitionoftheTh2phenotypebynaïveCD4+Tcells,primingandchemotaxisofmastcells,eosinophils,andbasophils,andtheproliferationandactivationofepithelialcells(10‑13).IL-4playsadominantroleinthedevelopmentofallergicinflammationandasthma(12,14).

  1. Benczik,M.andS.L.Gaffen(2004)Immunol.Invest.33:109.
  2. Chomarat,P.andJ.Banchereau(1998)Int.Rev.Immunol.17:1.
  3. Yokota,T.etal.(1986)Proc.Natl.Acad.Sci.83:5894.
  4. Redfield,C.etal.(1991)Biochemistry30:11029.
  5. Ramirez,F.etal.(1988)J.Immunol.Meth.221:141.
  6. Leitenberg,D.andT.L.Feldbush(1988)Cell.Immunol.111:451.
  7. Mosman,T.R.etal.(1987)J.Immunol.138:1813.
  8. Mueller,T.D.etal.(2002)Biochim.Biophys.Acta1592:237.
  9. Nelms,K.etal.(1999)Annu.Rev.Immunol.17:701.
  10. Paludan,S.R.(1998)Scand.J.Immunol.48:459.
  11. Corthay,A.(2006)Scand.J.Immunol.64:93.
  12. Ryan,J.J.etal.(2007)Crit.Rev.Immunol.27:15.
  13. Grone,A.(2002)Vet.Immunol.Immunopathol.88:1.
  14. Rosenberg,H.F.etal.(2007)J.AllergyClin.Immunol.119:1303.

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激酶是一类生物化学里的分子,从高能供体分子(如ATP)转移磷酸基团到特定靶分子(底物)的酶,这一过程谓之磷酸化。最大的激酶族群是蛋白激酶。 蛋白激酶作用于特定的蛋白质,并改变其活性。这些激酶在细胞的信号传导及其复杂的生命活动中起到了广泛的作用。其他不同的激酶作用于小分子物质(脂质、糖、氨基酸、核苷等等),或者为了发出信号,或者使它们为代谢中各种生化反应作好准备。
相关疾病:冠心病不稳定型心绞痛肿瘤腹痛患者.女,66岁,主因间断上腹痛半年,加重1月入院,入院查胃镜是胃多发息肉,非萎缩性胃炎,腹部ct子宫切除术后,右肾多发囊肿,副脾。既往有冠心病不稳定心绞痛,无支架,入院心电图正常,心脏彩超:二尖瓣......
相关疾病:血栓形成请教各位大神,请教一下:尿激酶能否被血液透析所清除,目前查了一些文献,找不到具体的。长期导管存在血栓形成和纤维套鞘,有时候常规尿激酶封管、尿激酶溶栓效果可能不佳,在透析中出现血流不足,可否边透析边滴注......

2013dialysisofdrugs.pdf(170.37k)
肌酸激酶同工酶的基本形式123
幻世萌zedit2018-01-17
中文名称:血清磷酸肌酸激酶
肌酸激酶有四种同功酶形式:肌肉型(MM)、脑型(BB)、杂化型(MB)和线粒体型(MiMi)。MM型主要存在于各种肌肉细胞中,BB型主要存在于脑细胞中,MB型主要存在于心肌细胞中,MiMi型主要存在于心肌和骨骼肌线粒体中。肌肉型肌酸激酶分子是由两个相同的亚基组成的二聚体。根据目前已经测定的兔、人、鸡、鼠肌酸激酶的一级结构[3-6],M型亚基由387个氨基酸残基组成,质量为43 KDa左右,分子内有8个巯基,但无二硫键。大熊猫肌肉型肌酸激酶也是二聚体酶,每个亚基由376个氨基酸残基组成,分子量为42 KDa[7]。向左转|向右转
5蛋白激酶与磷酸酶 123
yangliang_neau2021-07-22
蛋白激酶不是也是磷酸化蛋白的吗?与蛋白磷酸酶有什么区别呢?
人体内多种组织和器官内含有肌酸激酶,其分布不均.主要分布在骨骼肌的肌纤维内.因此,运动容易使血清肌酸激酶活性升高,其原因是因为运动时缺氧,代谢产物堆积,供能相对不足等原因引起肌细胞膜通透性升高,或肌细胞膜受到损伤,促使酶从细胞内释放增加,酶进入血液循环,其中尤以肌肉牵拉的机械性损伤或产生血肿等原因较为重要.不同的运动负荷,包括长距离自行车,滑雪及马拉松跑对机械性影响较大,可以导致血清肌酸激酶的活性升高,特别是大强度的马拉松跑后4小时后开始升高,8小时达高峰.跳跃,短跑等短时间激烈运动是冲击性运动,也引起血清肌酸激酶活性升高.运动员在比赛后,肌酸激酶可上升至734 IU/L左右(正常值200IU/L以下),与急性心肌梗死病人数值相近,但运动员血清肌酸激酶来自骨骼肌.为了避免运动后血清肌酸激酶的升高,检查血清肌酸激酶时应避免激烈的运动,以免临床误诊.以下是CPK增加的原因,也是临床诊断的依据:1.各种类型肌营养不良均可发生CPK活力升高,其中假肥大型肌营养不良酶的活力约可达正常的10--50倍.2.肌肉创伤可使CPK活力升高.3.剧烈运动,手术,肌肉注射也可引起此酶活性升高.4.脑血管意外,脑膜炎,甲状腺机能低下等也可使CPK活力升高.5.急性病毒性或风湿性心肌炎时CPK活性可达正常时的5倍.6.急性心肌梗时,早期阳性检测率高,特异性强,酶活力上升程度与疾病严重程度相一致.wanghanlin9852010-2-3 22:31:51您好:您肺部疾患现在没有完全确诊,肌酸激酶增高根据您的病情考虑心脏受累,建议查下心脏彩超有无器质性改变,根据病情在进行治疗.
病情分析:肌酸激酶 CPK 主要存在于骨骼肌,心肌中,对急性心梗有较高的诊断价值,正常值 男 24~170U/L 女 24~150U/L意见建议:心肌梗死后CK在12~48达到高峰,这个时候检查比较有意义
酪氨酸激酶可分为三类:①受体酪氨酸激酶,为单次跨膜蛋白,在脊椎动物中已发现50余种;②胞质酪氨酸激酶,如Src家族、Tec家族、ZAP70家族、JAK家族等;③核内酪氨酸激酶如Abl和Wee。
根据PTK是否存在于细胞膜受体可将其分成受体型和非受体型。向左转|向右转

大家好,有几个设计的药物分子需要测试活性,不知道谁能提供下能做相关测试服务的机构或个人信息不?非常感谢!

纳豆是黄豆发酵后的食物,而纳豆激酶则是在发酵过程中产生的蛋白酶。
  1、纳豆激酶是在纳豆发酵过程中由纳豆枯草杆菌(Bacillus subtilisl natto)产生的一种丝氨酸蛋白酶(单链多肽酶),分子量为27728道尔顿。
  2、纳豆激酶在温度超过80℃时迅速变性失活,但反复冻融对其影响不大。
  3、纳豆激酶在PH值从7升至12时,10min内稳定;PH值低于5时,迅速变性失活。胃酸环境中的PH值只有1.2到2之间,纳豆激酶根本无法通过。
  4、纳豆激酶与粘性物质混合后,在PH值2-3的酸性环境中,还能保持不超过7.5%的活性。
  5、纳豆激酶是大分子的单链多肽酶,可被肠道消化液(糜蛋白酶、胰蛋白酶、小肠液等)分解成氨基酸片段或分子量更小的肽链。
  纳豆一词源于日本,是日本发酵食品,源自中国的咸豆豉,但并非中国豆豉,而是由黄豆通过纳豆菌(枯草杆菌)发酵制成豆制品,具有黏性,气味较臭,味道较甜,不仅保有黄豆的营养价值、富含维生素K2、提高蛋白质的消化吸收率,更重要的是发酵过程产生了多种生理活性物质,具有溶解体内纤维蛋白及其他调节生理机能的保健作用。
相关疾病:心肌梗死肿瘤心肌缺血心肌炎心律失常患者老年男性,发热伴咳嗽咳痰入院,入院后查心肌酶谱肌酸激酶明显偏高,而肌酸激酶同工酶正常,三天检查三次心电图均正常,没有心肌炎或者心肌梗死的症状,该如何分析这种情况,谢谢各位老师答疑解惑......
蛋白激酶(protein kinases)又称蛋白质磷酸化酶(protein phosphakinase)。一类催化蛋白质磷酸化反应的酶。
根据磷酸化的底物不同,可将蛋白激酶分为组蛋白蛋白激酶、酪蛋白蛋白激酶等,但由于蛋白激酶可磷酸化底物的多样化,这种分法很不确切,已经被根据底物磷酸化氨基酸的分类方法所取代,有些如酪蛋白蛋白激酶,只是由于习惯而一直被沿用下来。根据有无调节物将蛋白激酶分为信使依赖的蛋白激酶和非信使依赖的蛋白激酶,有些信使依赖的蛋白激酶的首字母缩略词已为人们所接受,如cAMP依赖的蛋白激酶PKA、钙和磷脂依赖的蛋白激酶PKC以及钙依赖钙调素不依赖的蛋白激酶CDPK等,它们彼此间存在结构和功能上的相关关系。

也有人认为:蛋白激酶在信号转导中主要作用有两个方面:其一是通过磷酸化调节蛋白质的活性,磷酸化和去磷酸化是绝大多数信号通路组分可逆激活的共同机制,有些蛋白质在磷酸化后具有活性,有些则在去磷酸化后具有活性;其二是通过蛋白质的逐级磷酸化,使信号逐级放大,引起细胞反应.