Broad Spectrum | iMFectin Poly DNA Transfection Reagent is powerful tool in delivering DNA to various established cell lines as well as primary cells. |
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Low Tocxic to Cells | iMFectin Poly DNA Transfection Reagent is very gentle on cells since it requires low amounts of reagents during transfection. This low toxicity enables a rapid protocol to transfect DNA into cells at the time of splitting or seeding. |
FBS & Antibiotics Compatible | High serum level with antibiotics usually do not have inhibitory effect on transfection efficiency. Expensive serum free media such as Opti-MEM are not required. |
Application | Exceptional high titers of virus production |
High levels of recombinant protein production | |
Good for very long DNAs (>89 kb) | |
Good for multi DNA co-transfection | |
Good for single DNA transfection |
iMFectin Poly DNA Transfection Reagent isa powerfulgene delivery compound, which enablesthe achievementof higher efficiencies inthe deliveryof DNA intoeukaryotic cell lines andprimary cellscompared toother DNA transfectionreagents.
This innovative transfection reagent containsa blendof biodegradable polymer based deliverycompounds,which facilitate transfection ofnucleic acid withoutany toxic effect.
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现在急用,但犹豫中,不知道他们的药盒效果怎样。有没有哪位同学用过啊?大家做放免的话是不是都是用解放军总医院的,还有别的好的研究所推荐吗?谢谢了
一般来说,试剂盒曲线上的最小浓度是比较准确的,低于这个值因为与曲线是个“S"型结构有关,所以结果是有偏差的,是一种估算。再加上实验误差,所以达不到理想的效果。建议选择试剂盒时,应该看曲线上最小的浓度值,而不是试剂盒上写的灵敏度。
虽然酶活性调节ELISA方法的灵敏度目前并不十分理想,但在酶活性放大ELISA中,检测的灵敏度远比RIA高。根据质量作用定律。即免疫反应所形成的免疫复合物量与反应物浓度成正比。推测所检测的待测物分子数为1。已知1个摩尔浓度含6.02×1023个分子,那么理论推测酶活性放大ELISA方法的最低检测限可达1.7×10-24mol/L。虽然在实际应用中由于反应条件和试剂纯度以及仪器精度等因素的影响,往往达不到这个水平(大于104个分子),但表明ELISA在灵敏度方面的改进潜力是很大的。
人FSH放免测试单位浓度为mIU/ml,可否换算成ng/ml,如何换算
非常感谢
希望知道如何购买的朋友告诉一下,谢谢。
我使用的是北京北方生物技术研究所提供的KIT.盒子里面有睾酮标准品,兔抗睾酮抗体,带碘标记的睾酮,和驴抗兔免疫分离剂。实验过后我查了一下生物化学书,发现睾酮这种激素的化学本质非蛋白质,而是缁醇。这样问题就出来了:据我所知,产生免疫反应的是蛋白质,然而性类固醇激素的化学本质是缁醇。请问各位,缁醇也能产生免疫反应吗?
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