Product Name | Fluorescein - 5 - maleimide |
CAS | 75350-46-8 |
Size | 25 mg |
Catalog # | AS-81405 |
US$ | $167 |
Purity | ≥95% by HPLC |
Maleimides are among the most frequently used reagents for thiol modification.In most proteins, the site of reaction is at cysteine residues that either are intrinsically present or result from reduction of cystines. Unlike iodoacetamides, maleimides do not react with histidines and methionines under physiological conditions. Fluorescein-5-maleimide is one of the most popular fluorescent dyes for thiol modifications of proteins. | |
Detailed Information | DatasheetMaterial Safety Data Sheets (MSDS) |
Storage | -20°C desiccated and protected from light |
References | 1. Winograd E, et al. Mol Biochem Parasitol 136, 243-8; (2004).2. Van der Sluis EO, et al. FEBS Lett 527, 159-65; (2002). |
Molecular Weight | 427.36 |
Molecular Formula | C24H13 NO7 |
Spectral Properties | Abs/Em = 493/515 nm |
Solvent System | DMF or DMSO |
Product Citations | Newbern, J. et al. (2007). c-Jun N-terminal kinase signaling regulates events associated with both health and degeneration in motoneurons. Neurosci 147, 680.Kamaduri, H, er al. (2013). Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3. elife2, e00828. doi: 10.7554/eLife.00828.Newbern, J. et al. (2007). c-Jun N-terminal kinase signaling regulates events associated with both health and degeneration in motoneurons. Neurosci 147, 680. |
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
两个意义不一样啊,定性是确定你抗体是阴还是阳的,如果阳证明2个情况,一个是你感染了丙肝病毒,另一个是你曾经感染过这个病毒,现在已经好了。但是到底是哪个情况还要进一步做定量测试既病毒RNA检测。如果这个测试值在最低线以下就证明你现在没事,不具有传染性也不是患者只是携带者;如果高于最低线那你就是患者了,就需要治疗
试剂盒里有详细的说明书,告诉你样品需要多少量,每个试剂需要加入多少量,和详细的实验步骤,一般买来就可以用,不用人教。
所以你问一个样需要多少量是没法回答的,测定过程是要加很多种试剂的。
定性ELISA只能粗略表示样本待测物含量在某个特定值以上或以下,定性ELISA通常设置阳性对照(P)、和阴性对照(N),结果分别用“阴性”和“阳性”表示。ELISA定性测定的“阴性”和“阳性”的判断标准是试剂盒所确定的阳性判断值,即cut-off值。
定量ELISA是通过一系列不同已知浓度的标准品所对应的OD值做出标准曲线,然后将样本的OD值代入标准曲线,计算出样本中待测物的含量。百特纯大分子Meretciel的定量ELISA试剂盒还可以。
暂无品牌问答