![SMOBIO/[QP3520] Q-PAGE™ Bis-Tris Precast Gel (Midi, 15 wells, 4-12%), 10 gels/Midi, 15 wells, 4-12%), 10 gels</span>
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Description
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Tris-Glycine gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
User-friendly gel cassette:
Numbered and framed wells for sample loading
With cassette opener for easy use
Enhanced gel performance:
Enhanced band sharpness
Better resolution of small proteins
Stable for shipping at ambient temperature
Easy compatibility:
Available as homogeneous and adjusted gradient gels for a wide range of protein separation.
Compatible with most popular protein electrophoresis systems
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels Remove tape and comb before electrophoresis.
Technical
Clear and sharp bands, high resolution
Q-PAGE™ Bis-Tris Precast Gel shows high resolution of protein separation.
QP3520 Specifications
Gel | Bis-Tris | |
Buffersystems | MOPS and MES | |
Features | Clear andsharp bands, highresolution | |
Cassettesize | Midi Gel (10 X 10 cm) | |
Geldimensions | 8.1 x 8.1 x0.1 cm (W x L xthickness) cm | |
Electrophoresissystem | Mini Gel Tank XCell SureLock, Hoefer SE260 | |
Well format& Capacity | 15 wells, 28 μl/well | |
Gelpercentage | 4-12 % | |
Accessorytray | Productiondescription Tip card Gel remover Cassetteopener | |
Manual
Manual_Q-PAGE™ Bis-Tris Precast Gel, Midi
SDS
SDS_Q-PAGE™ Precast Gel
Migration pattern
Setting Up and Running Q-PAGE™ Midi Precast Gel
Removing Q-PAGE from cassette
Setting up gel/membrane sandwich for Western transfer
Recommendations/Tips for Gel Running
1. Remove comb and tape before adaption. 2. Use fresh 1X running buffer for the inner cathode chamber. 3. Do not use Tris-Glycine running buffer for Q-PAGE™ Bis-Tris Precast Gels. 4. Rinse the wells before sample loading.
Sample Preparation for SDS-PAGE
1. Mix protein sample with 2X sample buffer.
2. Heat the diluted samples at 95°C for 5 min or at 70°C for 10 min.
3. Cool the diluted samples to 4°C and spin down the water condensed on tube surface. (If there is high viscosity part at bottom of tube, transfer supernatant to a new tube.)
Prepare Q-PAGE™ for Sample Loading
1.Open the blister tray of Q-PAGE™ Precast Gel.
2.Briefly rinse the gel cassette with ddH2O.
3.Remove tape and comb; avoid squeezing the gel.
4.Adapt Q-PAGE™ to electrophoresis system; instruction is provided below. (Invitrogen® Mini Gel Tank is recommended.)
5.Use a pipette to gently wash the wells with running buffer to remove residual storage buffer.
6.Fill the wells with running buffer prior to sample loading.
7.Load samples and pre-stained protein marker into numbered wells.
8.Fill both inner and outer chambers with running buffer to the highest level. Ensure gel wells are completely covered.
Power Setting for Running Q-PAGE™
Optimize the voltage and running time if needed.
| 130 V | 180 V | 230 V*2 |
Running Time*1 | 60-75 mins | 35-50 mins | 25-40 mins |
Expected Current Initial (per gel) Final (per gel) |
70-80 mA 20-30 mA |
90-100 mA 35-45 mA |
130-140 mA 60-70 mA |
Expected temperature | 25-30°C | 25-35 °C | 35-45°C |
*1 Set voltage higher than 100 V is recommended.
*2 For higher voltage conditions, please use fresh running buffer for inner and outer chambers.
*3 Running time varies depending on gel percentage, running buffer, temperature, and power supply.
Remove Q-PAGE™ Midi Gel from Cassette
Open cassette immediately after electrophoresis. Avoid gel drying.
1.Insert the cassette opener into corners of cassette.
2.Sequentially pry the opener to separate the two plates.
3.Gently pull up notched plate and let gel stay on the front plate.
4.Use cassette opener to push through the slot in the cassette.
5.Carefully detach the gel from the bottom of gel
- Avoid diagonally peeling the gel from the corner.
- If necessary, cut well separators with gel remover
6.Gently remove the gel for further staining or Western blotting.
Gel Staining
Proteins separated using Q-PAGE™ Precast Gels can be further stained with most popular staining reagents, such as Coomassie dyes (R-250 or G-250), Silver-stain solution,
and FluoroStain™ Protein Fluorescent Staining Dye. (Cat. No. PS1000)
Transferring Protein from Q-PAGE™ to Blotting Membrane
1. After protein separation using Q-PAGE™, gently detach QPAGE™ from cassette and then equilibrate the gel in transfer buffer.
2. Pre-soak blotting membrane and filter papers in transfer buffer.
*Activate PVDF membrane in methanol before soaking in transfer buffer.
**Prepare 6 filter papers for one gel/membrane sandwich.
3. Assemble transfer sandwich by orientating cathode, sponge, filter papers, gel, membrane, filter papers, sponge, and anode. The protein goes to the direction of cathode to anode.
4. Carefully move roller over the gel/membrane to remove air bubbles and excess buffer until complete contact is established.
5. Insert transfer cassette into transfer module. Notice that black side of cassette should be next to black side of module.
6. Fill transfer tank with pre-cooled transfer buffer to the highest water level.
7. Set constant voltage at 100 V. Transfer for 90 minutes at low temperature condition. Pre-stained protein marker should be visible on the membrane after transfer is completed.
Transfer of proteins to the membrane can be checked using Ponceau S staining before blocking step.
Supplemental Information for Using Q-PAGE™ Precast Gel
Adapting Q-PAGE™ Midi Precast Gels to Invitrogen Mini Gel Tank Electrophoresis System
1. Place the Q-PAGE Midi Precast Gels with notched plate facing toward yourself. No extra adapter is needed.
2. Seat the gels on the bottom of Mini Gel Tank and close the cassette clamp.
3. Fill chambers with running buffer to the level of the fill line. Ensure gel wells are completely covered.
Adapting Q-PAGE™ Midi Precast Gels to other electrophoresis system, please follow the manufacturer’s instruction.
Buffer recipes
2X sample buffer with reducing agent
62.5 mM Tris-HCl pH 6.8, 2% SDS, 25% (v/v) glycerol, 0.01% bromophenol blue, 5% β-mercaptoethanol or 100 mM DTT (added fresh)
10X MOPS running buffer
60.6 g Tris base, 104.6 g MOPS, 10.0 g SDS, 3.0 g EDTA. Bring up the volume to 1 L with ddH2O.
10X MES running buffer
60.6 g Tris base, 97.6 g MES, 10.0 g SDS, 3.0 g EDTA. Bring up the volume to 1 L with ddH2O.
1X running buffer
Dilute 100 ml 10X running buffer with 900 ml ddH2O.
10X transfer buffer
30.0 g Tris base, 144.0 g Glycine. Bring up the volume to 1 L with ddH2O.
1X transfer buffer
*Cool 1X transfer buffer to 4°C before using.
Dilute 100 ml 10X transfer buffer with 200 ml methanol and 700 ml ddH2O.
**Add SDS to 0.1% to promote transfer of high molecular weight proteins.
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Q-PAGE™ Precast Gel
Gel Type | Bis-Tris | TGN (Tris-Glycine-Novel) | ||||||
Buffer systems | MOPS and MES | Tris-Glycine (Laemmli) | ||||||
Features | Clear and sharp bands, high resolution | Quick running, clear bands | ||||||
Cassette size | Mini Gel(10 x 8.3 cm) | Midi Gel(10 X 10 cm) | Mini Gel(10 x 8.3 cm) | Midi Gel(10 X 10 cm) | ||||
Electrophoresis system | Bio-Rad systems | Mini Gel Tank Xcell SureLock, Hoefer SE260 | Bio-Rad systems | Mini Gel Tank Xcell SureLock, Hoefer SE260 | ||||
Well format & Capacity | 12 wells, 25 μl/well | 15 wells, 22 μl/well | 12 wells, 40 μl/well | 15 wells, 28 μl/well | 12 wells, 25 μl/well | 15 wells, 22 μl/well | 12 wells, 40 μl/well | 15 wells, 28 μl/well |
Gel percentage/ Cat. No. | 8% | 8% | 8% | 8% | 10% | 10% | 10% | 10% |
QP2110 | QP2120 | QP3110 | QP3120 | QP4210 | QP4220 | QP5210 | QP5220 | |
12% | 12% | 12% | 12% | 4-15% | 4-15% | 4-15% | 4-15% | |
QP2310 | QP2320 | QP3310 | QP3320 | QP4510 | QP4520 | QP5510 | QP5520 | |
4-12% | 4-12% | 4-12% | 4-12% |
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QP2510 | QP2520 | QP3510 | QP3520 |
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ExcelBand™ Protein Markers
Ready-to-use— premixed with a loading buffer for direct loading, no need to boil
Broad range— 310 kDa to 5 kDa
Pre-stained bands — for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Enhanced bands— for quick reference
YesBlot™ Western Marker I
Ready-to-use — no need of mixing or heating before sample loading
Direct visualization — 10 IgG-binding proteins for direct visualization on Western blots
Pre-stained bands — 4 pre-stained proteins for monitoring protein separation during electrophoresis and Western blotting transferring efficiency on membrane
Wide range — 10 clear bands from 15 to 200 kDa for size estimation
Quick reference — two enhanced bands (30 and 80 kDa)
FluoroStain™ Protein Fluorescent Staining Dye
Compatible to MASS analysis — compatible to the analysis of mass spectra, such as LC-MS/MS, MALDI-TOF, and etc.
High sensitivity — detection level achieve ~3 ng, similar to silver staining
Substitution of the Coomassie Blue protein staining method
ebiomall.com
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往常一直用“猴年马月”来形容时间的漫长，来看看我们身边与“猴”相关的药物物种都有哪些呢？您最熟悉的是什么？
今天逛超市，印象最深的是据说有“抗癌”作用的猴头菌，来看看它的功效有哪些
《全国中草药汇编》：猴头菌
【药材名称】猴头菌
【别名】猴头、刺猬菌、猬菌
【来源】真菌类担子菌纲多孔菌目齿菌科猴头菌Hericiumerinaceus（Bull.exFr.）Pers.，以子实体入药。
【生境分部】生于栎、胡桃等阔叶树种的立木及腐木上。
【性味】甘，平。
【功能主治】利五脏，助消化。主治消化不良，神经衰弱、身体虚弱。
【用法用量】消化不良：猴头菌2两，水浸软，切成薄片，水煎服，日服2次，黄酒为引。
神经衰弱、身体虚弱：猴头菌（干品）5两，切片与鸡共煮食用，日服1次（或用鸡汤煮食）。
【备注】（1）据文献，本品子实体内含抗癌物质。
（2）还有一种小刺猴头菌Herieiumcaptmedusae（Bull.exFr.）Pers.，刺密而短，效用同猴头菌。
【摘录】《全国中草药汇编》
下面是《中华本草》的记载：
【药理作用】1．增强免疫功能
同基因骨髓移植55d后，受体小鼠免疫功能严重损害。脾细胞产生白介素-2（IL-2）能力等细胞免疫功能明显低下，连续腹腔注射猴头菌多糖和胸腺肽15d后，小鼠脾细胞产生IL-2能力及对刀豆素A（Con
A）刺激的增殖反应和混合淋巴细胞培养反应，均显着增强。说明猴头菌多糖与胸腺肽合用比单独使用胸腺肽更能明显促进同基因骨髓移植小鼠免疫功能的早期恢复。猴头菌多糖给小鼠腹腔注射2mg/只，连续7d，能明显提高小鼠腹腔巨噬细胞的吞噬功能；可促进溶血素生成，增加体液免疫的能力。若给小鼠腹腔注射2.88mg／只，连续8d，则可明显对抗由环磷酰胺中毒所引起的白细胞下降，下降率仅为对照组的一半。猴头菌多糖在体外对由ConA活化的小鼠胸腺细胞有较强的促进增殖作用，也可促进脾淋巴细胞的增殖，并对脂多糖（LPS）刺激的B细胞也有协同作用。
2．抑瘤作用在Swiss雄性小鼠左前腋皮下，接种肉瘤S180细胞，然后口服猴头菌多糖50mg／kg，100mg/kg，200mg／kg，每日1次，连续7d，结果表明，3个剂量组对荷瘤生长均有抑制作用；对自然杀伤（NK）细胞活性有明显的激活作用；荷瘤重量与其相应鼠脾NK细胞活性呈负相关猴头菌还能抑制黄曲霉素对大鼠的致肝癌作用，减少肝切面的病灶数。
3．抗溃疡作用及降血糖作用通过胃蛋白酶抑制吸附实验，证明猴菇菌片治疗胃溃疡的作用机制，可能是由于抑制胃蛋白酶活性而促进溃疡愈合。猴头菌多糖可降低小鼠正常血糖和四氢嘧啶所致糖尿病小鼠的血糖水平。
4．延缓衰老作用猴头菌丝体多糖和子实体多糖能显着增加果蝇飞翔能力，降低刚孵化果蝇和小鼠心肌组织脂褐质含量，并能增加小鼠脑和肝脏中超氧化物歧化酶（SOD）的比活力。
【性味】甘；平
【归经】入脾、胃经
【功能主治】健脾养胃;安神;抗癌。主体虚乏力;消化不良;失眠;胃与十二指肠溃疡;慢性胃炎;消化道肿瘤
【用法用量】内服：煎汤，10～30g，鲜品30～100g；或与鸡共煮食。
【各家论述】1.《临海水土异物志》：民皆好啖猴头羹，虽五肉臛不能及之，其俗言：宁负千石之粟，不愿负猴头羹CONG。
2.《农政全书》：如无花、麻姑、猴头之属，皆草木根腐坏而成者。
3.《新华本草纲要》：全草：味甘，性平。有利五脏、助消化、滋补、抗癌等功能。与本品有类似功效，且分布区相近似的尚有以下两个近缘种：玉髯
H.Coralloides(Scop．Fr.）Pers，exGtay及分枝猴头菌H.ramosum(Bull.Ex
Merat。）Letellier.
找了张照片，猴头菇哦
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日期：2016-04-28信息来源：分子医学研究所
北京大学分子医学研究所利用非人灵长类动物模型，发现盐皮质激素受体阻断剂可以治疗高血压伴随的血压昼夜节律紊乱，提出高血压治疗的新机制和新的药物评价指标。
高血压是临床上最多见的心血管疾病之一，常伴有包括心、脑、肾等多种器官的功能或器质性损害，严重威胁人类健康。血压的调控具有明显的昼夜节律，动脉血压一般在夜间入睡后降低10-20%，清晨迅速回升，而高血压患者血压的昼夜节律紊乱，尤其是夜间血压降低的幅度明显减小。临床证据表明，血压昼夜节律紊乱与病人心血管事件的发生率和死亡率密切相关，因此，恢复血压昼夜节律对高血压的治疗及预防其并发症的发生具有重要的意义。
自2006年以来，北京大学分子医学研究所非人灵长类研究中心利用恒河猴建立了多种代谢性和心血管疾病模型。本研究首次建立了自发性代谢综合征高血压恒河猴模型，而后利用手术植入血压无线遥测系统，对恒河猴的24小时血压进行长达数月的实时连续测量。结果发现，自发性代谢综合征高血压恒河猴模型不仅血压升高，而且夜间血压降低的幅度也显著变小。利用一线临床抗高血压药物伊普利酮（eplerenone），不仅降低了血压，而且明显恢复了夜间血压降低的幅度，改善了血压昼夜节律的紊乱。同时伊普利酮还降低了血液中炎症因子和糖基化终末产物的浓度。
本研究首次在灵长类动物模型上证明了改善紊乱的昼夜血压节律也是伊普利酮治疗高血压、保护心血管系统的重要因素，同时发现了新的伊普利酮的作用机制。自发性代谢综合征高血压非人灵长类动物模型的建立，为临床高血压发病机制和药物治疗研究提供了非常重要的动物模型：与常用的啮齿类动物模型相比，非人灵长类动物模型在药物代谢、心血管疾病的发生和遗传等方面与人类更加类似；而与人类相比，该模型又具有不受药物和生活方式的干扰、研究条件可控性强等优势。
该工作于近日在线发表于ScientificReports杂志（http://www.ncbi.nlm.nih.gov/pubmed/?term=27032687）。张岩副研究员是本文的第一作者；张秀琴研究员和肖瑞平教授为文章的共同通讯作者。该项研究得到国家自然科学基金委、科技部“973”项目、科技部国家科技重大专项、北大-清华生命科学联合中心、生物膜与膜生物国家重点实验室、北京市重点实验室和默沙东公司的支持。
A图为伊普利酮治疗前后血压的昼夜变化B图为伊普利酮的功效
中新网2月26日电据美联社报道，科学家称已经***了猴子抵抗艾滋病毒感染的机密。这是一个让人欣喜的重大发现，因为接下来的研究很可能发现一个人类预防艾滋病的新策略。
某些灵长目动物可以防止艾滋病菌在体内蔓延，这一谜团困扰科学家长达10年之久，如今终于有了答案。
美国健康研究所基础科学研究室主任称，人们根据这一发现可能研制出治疗爱滋的药物，或者是预防爱滋的疫苗。“我们将立即就15个不同方向进行研究。这将是基础研究意义重大的一年，我们取得了很多的研究成果，而且很多工作还在继续。”
此项发现是由约瑟夫.缲德劳斯克博士和他领导的哈佛大学研究人员首先公布的。约瑟夫现在波士顿达纳法伯癌症研究所工作。报告结果发表在2月27日《自然》杂志上。
艾滋病毒的发病原理是病毒先进入细胞内部，强占细胞组织，并用来不断进行自我复制。但是猴子体内有一种名为TRIM5-alpha的蛋白成分，可以阻止艾滋病毒进入健康细胞后脱落自身保护层。虽然对于病毒保护层的脱落问题还有待进一步认识，但是科学家认为这是艾滋病毒感染周期的一个重要环节。
加利福尼亚大学一位研究艾滋病的微生物专家称，人类体内也有名为TRIM5-alpha的成分，但是抵抗艾滋病毒的能力远远不如猴子体内的同类成分。
哥伦比亚大学一位生物化学家称，“由于这个报告的出台，各个大学的实验室都将就此问题进行研究。”
缲德劳斯克博士称在动物身上还可能找到治疗其它病毒的成分。“我们发现的也许只是自然预防系统中的第一个例子，通过同样的方法，我们很可能找到对抗其它病毒的成分。”
艾滋病毒是一种逆转录酶病毒，它可以把基因信息写入感染细胞当中，并且进行不断的复制。所以一旦感染，就很难根除。但是逆转录酶病毒也有弱点，它们如果一开始找不到立足点的话，就会很快消亡。所以TRIM5-alpha蛋白有助于在感染的初始阶段阻止病毒的蔓延。“我们知道艾滋病毒也可以感染猴子的细胞，但是感染一开始就被控制住或者终止了。只是我们还不清楚具体的控制时间。”
如题，哪几家做定制猴抗体服务比较好的？
谢谢
暂无品牌问答
