- Gap 27
- 10Panx
- Scrambled 10Panx
- Gap19
Gap 26Gap junction blocker peptide, mapping to connexin 43 residue 63-75 |
Sample solution is provided at 25 µL, 10mM.
- 1. Mederos S, Hernández-Vivanco A, et al. "Melanopsin for precise optogeneticactivation of astrocyte-neuron networks." Glia. 2019 Jan 11.PMID:30632636
- 2. Zhang X, Chen D, et al. "Involvement of sphingosine-1-phosphate receptors 2/3 in IR-induced sudden cardiac death." Heart Vessels. 2019 Jan 2.PMID:30604190
- 3. Condamine S, Lavoie R, et al. "Functional Rhythmogenic Domains Defined by Astrocytic Networks in the Trigeminal Main Sensory Nucleus." Glia. 2017 Oct 23.PMID:29058348
- 4. Yang G, Peng X, et al. "Involvement of connexin 43 phosphorylation and gap junctional communication between smooth muscle cells in vasopressin-induced ROCK-dependent vasoconstriction after hemorrhagic shock." Am J Physiol Cell Physiol. 2017 Oct 1;313(4):C362-C370.PMID:28974518
- 5. Li X, Jiang S, et al. "Breakthrough Cancer Pain Is Associated with Spinal Gap Junction Activation via Regulation of Connexin 43 in a Mouse Model." Front Cell Neurosci. 2017 Jul 17;11:207.PMID:28769766
- 6. Chu H, Huang C, et al. "Reduction of Ischemic Brain Edema by Combined use of Paeoniflorin and Astragaloside IV via Down-Regulating Connexin 43." Phytother Res. 2017 Jul 28.PMID:28752625
- 7. Zhou, Ziyi, et al. "Protection of erythropoietin against ischemic neurovascular unit injuries through the effects of connexin43." Biochemical and biophysical research communications 458.3 (2015): 656-662.PMID:25684187
Quality Control & MSDS
- View current batch:
- Purity = 99.56%
- COA (Certificate Of Analysis)
- HPLC
- MS (Mass Spectrometry)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Related Biological Data
Description | Gap26 (Val-Cys-Tyr-Asp-Lys-Ser-Phe-Pro-Ile-Ser-His-Val-Arg) is a connexin mimetic peptide, corresponding to residues 63-75 of connexin 43, which is a gap junction blocker. | |||||
Targets | ||||||
IC50 |
Cell experiment: [1] | |
Cell lines | ECV304 cells |
Preparation method | The solubility of this peptide in sterile water is >10 mM. Stock solution should be splited and stored at -80°C for several months. |
Reaction Conditions | 0.25mg/ml, 30min |
Applications | Preventing the InsP3-triggered calcium increase by ester loading the cells with the calcium chelator BAPTA reduced the InsP3-triggered ATP release back to the control level. Incubation of the cells with gap 26 completely abolished the InsP3-triggered ATP response and reduced the ATP release to below the control level, indicating that the basal ATP release is also affected. |
Animal experiment: [2] | |
Animal models | Female Sprague-Dawley rats |
Dosage form | 300 μM, 45 min |
Applications | The rats were prepared with closed cranial windows 24 h before the study. A 10-mm-diameter craniotomy was performed over the skull midline. The dura was removed carefully to keep the sagittal sinus intact. An 11-mm-diameter glass window outfitted with three ports was glued to the skull using cyanoacrylate. The skin overlying the window was sutured, and the animals were permitted to recover. On the day of study, three stainless steel screws were inserted into the skull, along the periphery of the cranial window, for electroencephalogram (EEG) recording. Cannulae were then connected to the three ports. The rats were subjected to one of two neuronal activation paradigms: SNS or bicuculline-induced seizure. Following the initial measurement of pial arteriolar diameter changes during SNS or during bicuculline exposure, baseline conditions were reestablished. After 20 min, a suffusion of gap-26 was initiated. Forty-five minutes later, the neural activation was repeated. Exposure to the Cx40/Cx37 inhibitory peptide, gap-26 (300 μM), was without effect on bicuculline- or SNS-induced pial arteriolar dilations. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1] Braet K, Vandamme W, Martin P E M, et al. Photoliberating inositol-1, 4, 5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26. Cell calcium, 2003, 33(1): 37-48. [2] Xu H L, Mao L, Ye S, et al. Astrocytes are a key conduit for upstream signaling of vasodilation during cerebral cortical neuronal activation in vivo. American Journal of Physiology-Heart and Circulatory Physiology, 2008, 294(2): H622-H632. |
Gap 26 Dilution Calculator
calculate
Gap 26 Molarity Calculator
calculate
Cas No. | 197250-15-0 | SDF | Download SDF |
Synonyms | Val-Cys-Tyr-Asp-Lys-Ser-Phe-Pro-Ile-Ser-His-Val-Arg | ||
Chemical Name | N/A | ||
Canonical SMILES | N/A | ||
Formula | C70H107N19O19S | M.Wt | 1550.79 |
Solubility | ≥77.55 mg/mL in DMSO with ultrasonic and warming, <7.75 mg/ml="" in="" etoh,="" ≥155.1="" mg/ml="" in="" h2o="" with="" ultrasonic="">7.75> | Storage | Desiccate at -20°C |
Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. |
Gap26 (Val-Cys-Tyr-Asp-Lys-Ser-Phe-Pro-Ile-Ser-His-Val-Arg) is a connexin mimetic peptide, corresponding to residues 63-75 of connexin 43, which is a gap junction blocker.
Connexins, or gap junctions, are a family of structurally-related transmembrane proteins. Gap junctions contain channels that allow the passage of ions and small molecules between adjacent cells molecules. Calcium and inositol phosphates are among the second messengers that can pass through gap junction channels. [1] It was showed that gap26 attenuates rhythmic contractile activity of rabbit arterial smooth muscle (IC50 = 28.4 mM). It also blocks movement of IP3-induced ATP and Ca2+ across connexin hemichannels, i.e. hexameric channels yet to dock with partners in aligned cells and to generate the gap junction cell–cell conduit. [2]
Fig. 1: Formula of Gap26
Fig. 2: Function of Gap26.
Ref:
1. Boitano, S. and H. Evans Am. J. Physiol. Lung Cell Mol. Physiol. 279, L623 (2000).
2. T. Desplantez, V. Verma, L. Leybaert, W.H. Evans, R. Weingart, Gap26, a connexin mimetic peptide, inhibits currents carried by connexin43 hemichannels and gap junction channels, Pharmacological Research, Volume 65, Issue 5, May 2012, Pages 546-552.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
各位大侠:小弟最近做个长片段的CDNA与T载体的连接,片段大小6.4K,载体选用的是pUC19和pGEMT-easy,反转录酶用的是RevertAidTirstStrandcDNASynthesisKit(#K1621),CDNA第二链的扩增用的是LATaq。我是用纯病毒的RNA做模板,操作步骤严格按照说明做的,也曾经有一次获得过电泳图清晰显示的是全长6.4KB左右的片段,与载体连接,结果筛选到的几个克隆,插入的片段仅有2KB左右,奈何?可最近几次再用此反转录试剂盒却不能得到所需片段。在此,小弟想问问大家,你们是否遇到同样的问题,是如何解决的?所用的试剂(载体和酶的选择)和方法是什么,可以告诉后来者,共同进步吧。
2. 禽成髓细胞瘤病毒(AMV)反转录酶:有强的聚合酶活性和RNA酶H活性。最适作用温度为42℃。
3.Thermus thermophilus、Thermus flavus等嗜热微生物的热稳定性反转录酶:在Mn存在下,允许高温反转录RNA,以消除RNA模板的二级结构。
4.MMLV反转录酶的RNase H突变体:商品名为SuperScript 和SuperScriptⅡ。此种酶较其它酶能多将更大部分的RNA转换成cDNA,这一特性允许从含二级结构的、低温反转录很困难的mRNA模板合成较长cDNA。向左转|向右转
暂无品牌问答