WesternBlottingHRPSubstrate
lumigenECLUltra(TMA-6)isaproprietaryacridanbasedchemiluminescentsubstrateforultra-sensitivedetectionofWesternblottedandELISAcapturedproteinsthatareboundtohorserADIshperoxidase(HRP)labeledantibodies.
- Veryrapidonsetofchemiluminescence- imagescanberecordedinseconds
- Highestsensitivity -midtolowfemtogram
- Reducedantibodyusage - morethan10-foldreductionintheusageofexpensiveantibodyconjugates
- Temperatureinsensitive - analyticalresultsareinsensitivetotemperaturesfrom22°C - 35°Creducingtheneedforprecisetemperaturecontrol
- Sustainedsignalduration - overseveralhoursallowsconvenientimaging
StableWorkingSolution
SimilartoLumigenECLExtra,LumigenECLUltrahasexcellentstABIlity.Theworkingsolutionisstableatroomtemperatureforatleast oneweekandat2-8°Cforatleast onemonth.Withthisstability,theworkingsolutioncanbepreparedandstoredforlateruse.
RapidPeakIntensity
ReactionofthesubstratewithHRPenzymegeneratesrapidluminescenceofthehighestintensitywithinseconds.Peakintensityoflightemissionoccursat440nm.
ProductSpecifications
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
转膜后就要区分与胶接触的一面与另一面了。话说楼主好奇的话可以跑蛋白的试试看哈。比如目的蛋白的胶用光滑面贴着胶,内参蛋白用粗糙面贴着胶一起转膜试试转膜结果看看呢。
1、如果是提取的总蛋白,然后做WB,用β-actin或者GAPDH做内参肯定是没有问题的,这是公认的东西。
2、如果用膜蛋白提取试剂盒提取蛋白,再用β-actin作为内参似乎不妥,因为理论上来讲β-actin在膜上是不表达的。WB能做出β-actin来是因为膜蛋白提取时把胞质蛋白也提出来了。然而,如果我们试验目的是用药物处理细胞,比较处理前后某种膜蛋白的表达情况,此时用膜蛋白提取试剂盒提取膜蛋白后再用β-actin做内参似乎就不妥了,因为你根本不知道药物处理前后混杂了多少的胞质蛋白进来。如果没有胞质蛋白混进去的话,β-actin就是检测不到的。