Catalog number | SM039G |
Product type | ELISA |
Quantity | 96 Tests (12x8 breakable strip wells) |
Sample volume | 10 µl/well |
Species | Human |
Storage and Stability | Product should be stored at 2-8 °C. |
Precautions | For research use only. Not for use in diagnostic procedures. |
References | 1. Tan, E.M. 1982. Autoantibodies to Nuclear Antigens (ANA) : Their Immunobiology and Medicine. Adv. Immunolo. 33 :167-240. 2. Nakamura, R.M., and E.M. Tan. 1986. Recent Advances in Laboratory Tests and the Significance of Autoantibodies to Nuclear Antigens in Systemic Rheumatic Disease. Clin. Lab Med. 6:41-53. 3. McCarty, G.A., D.W. Valencia, and M.J. Fritzler. 1984. Antinuclear Antibodies : Contemporary Techniques and Clinical Application to Connective Tissue Disease. In: Antinuclear Antibodies. Oxford Univ. Press, New York. pp 1-95. 4. Kurki, P., M. Gripenberg, P. Parlanen, and T. Helve. 1987. Screening Test for Rheumatic Disease: A Combined Enzyme Immunoassay of Rheumatoid Factors and Autoantibodies to DNA and Extractable Nuclear Antigens. J. Clin. Pathol. 40 : 1475-1480. 5. Geisler, C. and M. Hoier-Madsen. 1985. An Enzyme-Linked Immunosorbent Assay for Autoantibodies Against the Nuclear Protein Scl-70. J. Immuno. Methods. 80 : 211-219. |
SSA IgG ELISA
SSB IgG ELISA
RNP/Sm IgG ELISA
ANA IgG ELISA
dsDNA IgG ELISA
Scl-70 IgG ELISA
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用Mabteck公司的ALP标记抗体吧,配合Millipore公司的Multiscreen-IP96孔板,所有的试剂都可以减半,照样可以出非常好的结果,4*96孔的试剂盒我一直做8*96孔,但BCIP/NBT显色液没试过减半,因为它很便宜。记得当年第一次做实验就出了很好的结果,经过不断摸索,做出的蓝紫色斑点确实非常漂亮,几乎没有非特异斑点,嗯,ELISPOT是我最拿手的实验之一。
关于PBMC:
拿到血液标本后,首先我要做的是尽快分离PBMC(一般2h内),国产的分离液里天津灏洋的比较好,然后用冻存液冻存PBMC。PBMC的冻存过程比一般的细胞株要更为严格,最好用冻存器。ELISPOT试验的第一天,在包被抗体的同时,将细胞复苏,完全培养液培养过夜,第二天收集细胞并计数即可。
请问一般的实验室可以进行ELIspot吗?(我可以买到现成的试剂盒,资金这个应该没有问题。)ELIspot的难点在哪里?多谢大虾们的指点!这个对我很重要,多谢!!!
我想用酶联免疫斑点(ELISPOT)技术测定NK细胞活性,但不知道哪里能买到试剂盒,特向大家求助,谢谢。
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