Description
Oligo (dT)18 Primer is single-stranded sequence of deoxythymine (dT), used for priming reactions catalysed by reverse transcriptase. The transcript is primed in the poly(A) tail of mRNA molecules.
Product Highlights
- Long oligonucleotide -more thermostable Reverse Transcriptases perform better with longer primers
- Binds only to mRNA -can be used for cDNA synthesis with total RNA
Product Description
Oligo (dT)18 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)18 ensures that the 3´ end of mRNAs are represented.
Primer sequence: 5´-d (TTT TTT TTT TTT TTT TTT)-3´
A mixture of random hexamer primers and oligo(dT) may improve the sensitivity of cDNA synthesis.
Applications
- cDNA synthesis with a reverse transcriptase
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血液保存液属于医疗器械的几类?
如果需要储液箱,那储液箱和微型定量泵之间用什么连接?是用管道吗?
为了使供输血用的血液能保存一定时间,加入适当的抗凝剂,并尽量防止在保存期发生变质而用的液体。为了防止因糖的消失引起活力降低,尚加入葡萄糖。ACD液(含柠檬酸三钠、柠檬酸、葡萄糖)一般用于血库。添加ACD的血液放在4—6℃条件下保存,可保存三周。另外为防止柠檬酸盐的过量可用加磷酸钠的CPD液。此外还设计了加乳酸钠的ACDL液。如果加入甘油等防冻剂则可长时间冷冻保存红细胞。
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