Phoenix™ Hot Start Taq DNA Polymerase is a recombinant, thermostabile Taq DNA polymerase complexed with a thermolabile, neutralizing antibody that blocks the 5′→3′ polymerase activity prior to the initial DNA denaturation step of PCR (1,2). Such antibody-mediated Hot-Start capability enhances the overall specificity, sensitivity and yield of the PCR by reducing nonspecific amplification and primer-dimer formation prior to PCR cycling, and allows the convenience of reaction set up at room temperature. When the temperature of the PCR reaction mix reaches ≥94°C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored. Phoenix Hot Start Taq DNA Polymerase, like standard Taq DNA polymerase, also has 5′→3′ exonuclease activity, but lacks any detectable 3′→5′ exonuclease activity.

Source of ProteinA recombinant E. coli strain carrying the Taq DNA polymerase gene from the thermophilic organism Thermus Aquaticus YT-1 complexed with a monoclonal antibody derived from murine cell culture.

Supplied in20 mM Tris-HCl100 mM NaCl0.1 mM EDTAStabilizer50% GlycerolpH 7.5 @ 25°C

Supplied With:5X Phoenix Hot Start Taq Reaction Buffer (B7590)5X Phoenix Hot Start Taq GC Reaction Buffer (B7591)

Unit Definition1 unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Enzymatics专注于于酶产品的研发，为市场带来了特殊的、高品质的产品。是一家试剂、试剂盒和测定的领先生产商，使全球客户变革了诊断、治疗和生命科学的研究。业务包括：试剂、供应链解决方案，应用科学，其测序产品市场占有率达80%。

酶科技公司产品包括八类：超纯连接酶如T4连接酶、超纯poly酶、ArcherTM系列测序工具(ArcherProducts)、连接蛋白、DNA修饰酶、NGS文库制备(NGSLibraryConstruction)、核酸和RNA酶。

Enzymatics专注于于酶产品的研发，为市场带来了特殊的、高品质的产品。是一家试剂、试剂盒和测定的领先生产商，使全球客户变革了诊断、治疗和生命科学的研究。业务包括：试剂、供应链解决方案，应用科学。目前市场上80%测序产品来自Enzymatics（“Todayourproductsunderpinover80%ofthesequencingreactionsthatarerunaroundtheglobe”）。Enzymatics产品的好处之一--能提高效率：NatureMethods中提到“使用Enzymatics超纯连接酶，连接步骤的效率得以提高，连接片段的产量增加了20-30%”。好处之二--有效降低测序成本：遗传学界的泰斗GeorgeChurch更是认为Enzymatics是"apowerfulcatalystinthedemocratizationofsequencing"。合作伙伴包括：BGI，BerryGenomics，Illumina，454LifeSciences和IonTorrentSyst，皆对Enzymatics的出品赞不绝口。

Enzymatics--核酸25mM100µmoldNTPSolutionMix--N2050L,N2050F

核酸25mM100µmoldNTPSolutionMix

摘要：四种天然脱氧核苷酸的等摩尔混合溶液(25mM)

产品描述

Anequimolar(25mM)mixtureofthefournaturaldeoxynucleotides.

四种天然脱氧核苷酸的等摩尔混合溶液(25mM)

SuppliedAs:25mMeachdNTP(dATP,dCTP,dGTP,dTTP)atpH7.5

产品信息

PartNumberN2050L,N2050F

Concentration25mM

UnitSize100µmol,20µmol

产品特性

StorageTemperature-25to-15°C

Purity≥99%

BasePurity≥99.5%

Pyrophosphate≤0.003pmol/pmolofnucleotide

EnzScript™(MMLV逆转录酶，RNaseH-)

摘要：基于RNA的DNA聚合酶，无核酸内切酶活性

产品描述：

EnzScript™(M-MLV逆转录酶RNaseHminus)属于RNA依赖的DNA聚合酶，该酶无RNaseH活性。EnzScript™被用于从polyAmRNA或总RNA中产生首链CDNA以用于下游的一些用途，如RT-PCR,cDNA克隆或RNA-Seq的文库构建。RNaseH结构域中的点突变能增加酶的热稳定性，相比于野生型M-MLV逆转录酶，它支持全长转录物种产生更高的cDNA产量