- SynonymS100B,NEF,S100,S100beta
- SourceHuman S100B, His Tag (S1B-H5143) is expressed from E.coli cells. It contains AA Ser 2 - Glu 92 (Accession # NP_006263).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 11.5 kDa. The protein migrates as 11 kDa under reducing (R) condition (SDS-PAGE).
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human S100B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
- BackgroundS-100 protein beta chain (S100B),a member of the S-100 family, is also known as S-100 protein subunit beta and S100 calcium-binding protein B, which contains two EF-hand domains. S100B can weakly bind calcium but bind zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Also, S100B can bind to and initiate the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction,S100B may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Fuethermore,S100B could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. S100B may mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
- References
- (1)Yamaguchi F., et al., 2012, J. Biol. Chem. 287:13787-13798.
- (2)Smith S.P., et al., 1998, Structure 6:211-222
- (3)McClintock K.A., et al., 2003, J. Biol. Chem. 278:6251-6257.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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1、存在于细胞外环境时,不显复制活性,但保持感染活性,是病毒体或病毒颗粒形式。
2、抗原,是指能够刺激机体产生(特异性)免疫应答,并能与免疫应答产物抗体和致敏淋巴细胞在体外结合,发生免疫效应(特异性反应)的物质
流感病毒的基因组包含________基因,其中__________蛋白可作为__________的结构基础.
我不知道第一空如何填,第二空是否应该填"HA蛋白"?第3空是否应该填"突变"?
多谢各位啊!
新年快乐!
万事如意!
核心抗原由183个或185个氨基酸组成,高度磷酸化,是乙肝病毒核心颗粒的唯一结构蛋白。正由于它存在于Dane颗粒核心结构表面,被表面抗原覆盖,故不易在血循环中检出。核心抗原具有强免疫原性,可诱导很强的体液免疫和细胞免疫,刺激机体产生抗-HBc。
e抗原为可溶性蛋白质,传染性强,游离存在于血液中,虽然很早就被发现,在病理上认为是HBV复制以具有强感染性的一个指标,但其功能尚不清楚。抗-HBe的出现,是预后良好的征象。向左转|向右转
所以说,感染同一种病毒,每个人识别的表位可能不一样。
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