Highlights
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready:Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased:Complete RNA recovery without miRNA loss.
Description
Compatibility | TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®. |
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Equipment | Microcentrifuge, vortex |
Sample Inactivation | TRI Reagent® (provided with R2051, R2053) inhibits RNase activity and inactivates viruses and other infectious agents. |
Sample Source | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). |
Size Range | Total RNA ≥ 17 nt |
Yield | 50 µg RNA (binding capacity), ≥25 µl (elution volume) |
Q1: Is DNase I available for individual purchase?
All kit components are available for purchase separately.
Q2: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q3: Is the kit compatible with samples stored in DNA/RNA Shield?
Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.
Q4: Is Direct-zol suitable for very small numbers of cells?
Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)
Q5: Is it possible to extract proteins with the Direct-zol RNA kits?
Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.
Q6: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.
Q10: Which phenol-based reagents are compatible with Direct-zol?
The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.
Q13: I ran out of RNA Wash Buffer. Can I use something else?
Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”
-Adina B. (University of Guelph)
“This kit is amazing, I"ve got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“
-R.K. CSU
“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”
-H.Z. (Harvard Medical School)
Read MoreCat # | Name | Size | Price | |
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E1010-1-4 | DNA Digestion Buffer | 4 mL | $15.00 | |
E1010-1-16 | DNA Digestion Buffer | 16 mL | $29.00 | |
R2050-1-200 | TRI Reagent | 200 ml | $219.00 | |
R2050-1-50 | TRI Reagent | 50 ml | $70.00 | |
C1078-50 | Zymo-Spin IICR Columns | 50 Pack | $55.00 | |
R1003-3-12 | RNA Wash Buffer | 12 ml | $30.00 | |
R1003-3-48 | RNA Wash Buffer | 48 ml | $105.00 | |
E1010 | DNase I Set | 250 U | $56.00 | |
R2050-2-40 | Direct-zol RNA PreWash (Concentrate) | 40 ml | $42.00 | |
R2050-2-160 | Direct-zol RNA PreWash (Concentrate) | 160 ml | $166.00 | |
C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
R2050 | Direct-zol RNA Miniprep | 50 preps | $180.00 | |
R2052 | Direct-zol RNA Miniprep | 200 preps | $555.00 | |
W1001-6 | DNase/RNase-Free Water | 6 ml | $15.00 | |
W1001-30 | DNase/RNase-Free Water | 30 ml | $22.00 |
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RO逆渗透是一种通过目前国际流行的反渗透等办法,对原水进行过滤处理(物理法)后不添加任何化合物而生产出可供人类直接饮用的纯净水机器(也称为终端净水设备)。
采用水质符合中国卫生部《生活饮用水水质卫生规范》(2001)规定的市政自来水为原水,通过2个活性炭滤芯(1个颗粒活性炭、1个烧结活性炭)1个PPF溶喷滤芯对原水进行预过滤,再对预过滤水施加压力令其通过孔径大小为万分之一微米的RO(反渗透,英文Reverse Osmosis)膜,最后通过材质为果壳(椰壳)的载银活性碳(又名小T33)调节水的酸碱度(使制出的纯净水口感变得甘甜醇美)而生产出纯净水。
净水器使用砂滤、活性炭、超滤等技术,能在一定程度上净化水源。能去除泥沙、铁锈,但无法彻底去除水垢、细菌、病毒,属于初级过滤。使用KDF技术的净水器,能去除细菌、病毒,部分去除重金属,但无法去除水垢。
软水机基本都是采用离子交换技术,用钠型阳离子置换水中的钙镁离子(水碱),彻底去除水垢,可以给生活带来很多好处。
纯水机采用反渗透膜过滤技术,核心是微孔过滤,只有水分子和比水分子小的矿物质能通过膜并保留下来供饮用;细菌、病毒、水垢、污染物均不能通过膜,成为浓缩水排出;从而达到纯净水的标准。
主要看你具体的需要,
适合你的才是最好的。
我想咨询的是ELIX的处理柱(好象叫PRO-GUARD),多长时间换一次,我们这里在用水不是很大量的情况下半年就不行了,总是提示低压报警,现在刚换了三个月,又开始报警了,是否我们这里水质极差还是机器有问题?
该怎么解决呢,一个柱子近3000大洋,太贵了,还不如直接买纯净水划的来
一、净水机:
1、功能:能够过滤掉自来水氯、杂质等,最后出来的水能够达到生饮的标准,保留了自来水中的对人体有用的矿物质;
2、特点:没有电机,不需要电源,靠水压驱动过滤。没有储水罐,比较好的为五级过滤,第一级为滤芯,第二和第三级为活性炭,第四级为中空丝膜或陶瓷过滤,第五级为精致活性炭,主要用于改善口感。
二、纯水机:
功能:过虑掉自来水中所有物质,出来的水是纯净水,相当于我们买的纯净水,跟净水机相比,净水机只能过虑到杂质之类,纯水机还把水中的各种矿物质都过虑掉了,比如水里面对人体有害的各种重金属,净水机出来的水虽然有矿物质,但是只是自来水含有的,很多矿物质是自来水无法提供的,也无法过滤掉各种矿物质,如果希望纯水机出来的水含有矿物质,可以单加一个麦饭石,这样出来的就是矿物质比较丰富的矿泉水了,当然,这样的矿泉水肯定比净水机出来的可以生饮的水好。
纯水机的价格影响因素除了进口品牌和国产品牌的差异外,主要取决于产量、用途以及其他特殊要求。
综合来看,除非有硬性规定或者需要采用进口品牌来提升实验室形象,否则建议选择国产品牌即可,因为实际上纯水机作为实验室常规设备,在工艺技术上知名度较高的国产品牌已足与进口相媲美,且价格仅为进口的1/3左右。
你需要先确定采购水机的主要实验用途,不同的实验用途,对水的要求是不一样的,从而也决定了所采购纯水机的配置,进而决定价格。
水中需纯化的物质主要有:无机离子、总有机碳(TOC)、微小粒子、微生物(内毒素、热原)、弱电解质。根据对以上物质的去除度,生产出来的纯水分为三个级别:初级纯水、中级水、超纯水。一般来水,仅产初级产水的纯水机价格在1.2万左右(产量10L);同时产初级纯水和超纯水的纯水机价格在(2-3万左右,产量10L)。
此外,还需要确定具体的实验用途,不同的实验用水,对于水中的物质去除度是有区分的,会有一定的倾斜度,比如细胞培养型实验,就对无机离子和TOC去除度要求严格;无机分析实验的话对无机离子去除要求高。
国产中,锐思捷、力康都还不错。
暂无品牌问答