The human Interleukin-3 (IL-3) ELISABASIC kit is ideal for users who want the flexibility of setting up their own ELISA assay. This product enables quantification of human IL-3 in solution, e.g. in cell culture supernatant. The kit contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-ALP, ELISA standard and standard reconstitution buffer. Plates and substrate are not included.
Human IL-3 ELISABASIC kits are also available with Streptavidin-HRP. See Related products.
Serum/Plasma samplesAnalysis of serum/plasma samples with this kit requires the use of ELISA diluent for dilution of the samples, standard and detection antibody. The diluent prevents false positive read-outs which may be caused by interference of heterophilic antibodies found in serum and plasma. The ELISA diluent is available from Mabtech. See Related products.
ELISA substrateReady-to-use ELISA substrate is available in our web shop. See Related products.
Analyte Information
IL-3The cytokine Interleukin-3 (IL-3, IL3) is mainly produced by activated T cells. IL-3 is also called multi-lineage-colony stimulating factor since it induces the growth and differentiation of many cells. IL-3 is also an activator of mast cells, eosinophils, basophils and macrophages.
Ordering InformationOrders to Mabtech can be placed online, by email, or by phone/fax to any of our offices.
Email:Send your order to order@mabtech.com.
Customers from USA or Canada: Please send your order to mabtech.usa@mabtech.com.Application: |
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Reactivity: |
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Standard range: | 25-2500 pg/ml |
Documentation: | Datasheet/ProtocolSDS |
Contents: |
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Size: | for 6 plates |
Other formats: | Click to view all Human IL-3 products |
Very easy to use ELISA kit. |
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英 [ˈfɔ:mæt]美 [ˈfɔ:rmæt]
n. (出版物的)版式;[自](数据安排的)形式;电视节目的总安排(或计划)
vt. 使格式化;安排…的格局;设计…的版面
vi. 设计一个版式
流式细胞仪能检测多少个通道
需要同时检测检测多少个指标
厂家的抗体有多少种荧光标记
如果流式细胞仪的通道越多,那么同一份样本能同时做的表面/胞内标志就越多
A、 如何根据流式细胞仪搭配流式抗体
1) BD流式细胞仪
BD流式试剂选择及应用的相关问答与简介
流式细胞仪能检测多少个通道是由有多少根激光决定的以及每根激光的功能决定的,所以首先需要注意两点:
流式细胞仪有哪些激光。比如标配的FACSCalibur只有一根488nm的激光,能做FL1、FL2、FL3三个荧光通道/三个颜色,而选配的Calibur (FACSCalibur的简称)配有488nm和635nm两根激光,可以检测FL1-FL4四个通道/4个颜色。
不同型号的流式细胞仪的同样的一个通道检测荧光素的能力是不一样的,比如Calibur的FL3(第3通道)能检测PE-Texas Red, PE-Cy5, PerCP, PerCPCy5.5,PE-Cy7,而Aria的第3通道只能检测PE-Texas Red, PE-Cy5, PerCP。 Calibur第3通道能检测的PerCP-Cy5.5在FACSAria上是第4通道检测。Calibur和LSR都能检测4个颜色,但是第4个通道检测荧光素是不一样的
如果一个抗体可以用来“做western blot,免疫荧光,免疫共沉淀,ELISA”,我估计着是HRP或碱性磷酸酶或生物素之类标记的,然后需要再加荧光标记的二抗,这类抗体也是不能做流式用的。
我只用过流式的抗体来做免疫荧光(直接染色),效果还不错,但是反过来就没试过。
楼上两位还是要仔细看下产品说明书
尽量使用已经用荧光素标记好的单克隆抗体。
如果是单色实验,荧光素的亮度越强越好,比如标记了APC的抗体就要比标记了pacific blue的在相同抗原量,抗体用量相同的情况下,要亮很多
如果是多色实验,除了不要使用光谱重叠度高的荧光素以外,还要搭配染色指数。简单的说,就是用亮度强的荧光素标记的抗体来染水平低的抗原,而用较弱的荧光素标记的抗体来染高表达的抗原。
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