eIF4E Detection Kit
GloboZymes eIF4E Detection Kit includes 100 μl of rabbit Anti-eIF4E polyclonal antibody and an aliquot of highly purified recombinant human eIF4E. The eIF4E preparations are provided in SDS sample buffer ready for use in five applications as positive control in Western blots. The antibody is also effective for immunoprecipitation.
Synonyms: Eukaryotic Protein Synthesis Initiation Factor 4E Western Blot Detection Kit; eIF-4E Western Blot Detection Kit
Background: The mRNA Cap-binding protein synthesis initiation factor 4E (eIF4E) participates in an early rate limiting step in protein synthesis. Over-expression of eIF4E leads to cell transformation and tumorigenesis and occurs in a number of cancer cells. eIF4E occurs as an inactive complex with the 4EBP binding proteins in quiescent cells. However, in response to insulin and other extracellular stimuli, eIF4E dissociates from 4EBP and is recruited to the active eIF4F complex. In addition to eIF4E, the eIF4F complex contains eIF4A and eIF4G. In cells exposed to insulin and other growth factors, eIF4E is phosphorylated at Ser209. The affinity of eIF4E to capped mRNA is enhanced by this phosphorylation, which is catalyzed by the Mitogen-Activated Protein Kinase (MAPK) Interacting Kinases (Mnk’s). It is also catalyzed by an insulin-stimulated protein kinase termed cPK which also phosphorylates Thr210 on the initiation factor.
Figure: Western blot of purified preparation of eIF4E with Anti-eF4E.
References: J Biol Chem 270, 14824-14828; J Biol Chem 270, 14597-14603; Biochime 76, 822-830; J Biol Chem 271, 11831-11837; J Biol Chem 270, 21684-21688
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我一般用加样枪，直接在膜上来回涂，可是涂得很不均匀，有的时候还把膜划破损了。大家是怎么做的呢？？
钡离子焰色反应为黄绿色
钙离子燃烧砖红色
铷离子焰色反应为紫色
铜离子焰色反应为绿色
钠离子焰色反应为黄色
钾离子焰色反应为浅紫色
锶离子焰色反应为洋红色
经常使用这些元素的硝酸盐作为焰火发色剂。
有荧光为何还需要压片这么久呢？不知各位用的时候有无这种情况！
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