Antibody Enhancer
Cat. No. 4660
Antibody Enhancer is a signal enhancing buffer that can be used for the dilution, stabilization and binding capacity enhancement of concentrated antibodies and conjugated antibodies. The product secures molecular conformation and prevents loss of activity, of the diluent protein, over time.
Antibody Enhancer enables users to create prediluted ready-to-use conjugated antibodies (free poly- and monoclonal antibodies, HRP-conjugated antibodies, AP-conjugated antibodies and fluorescence-conjugated antibodies):
- Reduces background and provides highest signal-to-noise ratio due to the antibody’s enhanced binding capacity.
- Amplifies 40-80% the specific antibody’s activity without increasing the non-specific binding.
- Allows for the diluted conjugate to be stored at least 4 years at 2-8 °C.
- Minimizes assay errors related to daily dilution variation.
- Limited or expensive conjugates can be further diluted without losing sensitivity.
- Designed for use in colorimetric as well as in chemiluminescence based assays such as ELISA, Western Blotting and IHC.
The product is available in bulk quantities.
Specifications
- Available sizes:100 mL, 500 mL, 1 L, bulk
- Format:Ready-to-use (RTU)
- Storage:2-8⁰C
- Stability:4 years
Downloads
Download your LOT specific Certificate of Analysis (CoA)
ECO-TEK
- Free of Toxic Preservatives
- Free of Bovine Serum Albumin
- Free of Harmful Organic Solvents – No NMP
- Free of Hazardous Stop Solutions
ebiomall.com
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② 应取pH8.0,这样可使核苷酸带较多负电荷,利于吸附于阴离子交换树脂柱。虽然pH 11.4时核苷酸带有更多的负电荷,但pH过高对分离不利。
③ 当不考虑树脂的非极性吸附时,根据核苷酸负电荷的多少来决定洗脱速度,则洗脱顺序为CMP>AMP> GMP > UMP,但实际上核苷酸和聚苯乙烯阴离子交换树脂之间存在着非极性吸附,嘌呤碱基的非极性吸附是嘧啶碱基的3倍。静电吸附与非极性吸附共同作用的结果使洗脱顺序为:CMP> AMP > UMP >GMP。
此外,我们常常用“电泳法”判断液体的性质,是胶体还是溶液。在高中化学中我们就用过这种方法判断给定的液体是否为胶体。向左转|向右转
我使用的是1%琼脂糖凝胶,电泳缓冲液为0.5×TBE。
请问各位大虾,在不影响实验结果的前提下,这样的含EB的电泳缓冲液可以反复使用多少次?
谢谢^_^
2.配好的EDTA不灭菌行不?放4度冰箱等过完年回来直接拿去配TAE
3.TAE配好后不用灭菌吧
如果你是制胶或者做电泳缓冲液的话,不用灭菌。
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