Effect Diluent High
Cat. No. 5090
The Effect Diluent is an effective buffer for the dilution of serum, plasma, blood, stool or urine samples, as well as the dilution of primary and secondary antibodies. It efficiently minimizes cross-reactions and unspecific binding in immunoassays like ELISA, EIA, RIA, Western Blotting, Immuno-PCR, Protein arrays, multianalyte immunoassays and Immunohistochemistry.The Effect Diluent is used alternatively to the standard sample or antibody dilution buffer:
- In ELISA for the dilution of specimen and detection antibodies.
- In Western Blotting for the dilution of primary and secondary antibodies.
- In IHC for the dilution of primary and secondary antibodies.
- In Protein arrays for the dilution of specimen and detection antibodies.
Three versions of the Effect Diluent are offered: Low, Medium and High for optimal discrimination between specific and unspecific reaction and for minimizing strong interference effects e.g. by RF (rheumatoid factors), HAMAs (human-a-mouse Abs) or by endogenous components that bind and mask the analyte.
This product is not for sale in the US.
Specifications
- Available sizes:500 mL
- Format:Ready-to-use (RTU)
- Storage:2-8⁰C
- Stability:3 years
Downloads
Download your LOT specific Certificate of Analysis (CoA)
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
蛋白质上样缓冲液中的SDS和DTT分别可以屏蔽蛋白质电荷以及破坏二硫键,避免形成多聚体。
DNA上样缓冲液主要是起沉降和指示作用,不可用于蛋白,否则会影响蛋白相对分子量定量。
同样蛋白上样缓冲液中的tris-HCl浓度过高也会使溴酚蓝条带扭曲。
配制分三步: 1) 1 M Tris-HCl (pH 8.0) 50 ml的配制:称取Tris碱6.06 g,加超纯水40 ml溶解,滴加浓HCl约2.1 ml调pH至8.0,定容至50 ml。
蛋白质上样缓冲液中的SDS和DTT分别可以屏蔽蛋白质电荷以及破坏二硫键,避免形成多聚体。
DNA上样缓冲液主要是起沉降和指示作用,不可用于蛋白,否则会影响蛋白相对分子量定量。
同样蛋白上样缓冲液中的tris-HCl浓度过高也会使溴酚蓝条带扭曲。
暂无品牌问答