SDS-PAGE of DbpB Protein. Lane 1: Molecular Weight Marker. Lane 2: DbpB Protein. Load: 10 µl at 1:2 dilution. Predicted/Observed size: 60.3 kDa for DbpB-MBP (17.9 kDa for DbpB and 42.4 kDa for MBP).
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BORRELIA BURGDORFERI SENSU STRICTO (B31) DBPB PROTEIN
Borrelia burgdorferi DbpB recombinant protein, fused to an MBP-tag and produced from E. coli (>90% purity).
PRODUCT DETAILS – BORRELIA BURGDORFERI SENSU STRICTO (B31) DBPB PROTEIN
- Recombinant Borrelia burgdorferi sensu stricto (B31) DbpB protein (NCBI Accession Number: NP_045698.1).
- Greater than 90% pure (by SDS-PAGE) in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide.
BACKGROUND
Decorin Binding Protein B (DbpB) is encoded by the spirochete B. burgdorferi, which is carried by Ixodes ticks. Strain B31 is the type strain (ATCC 35210) for this organism and was derived by limited dilutional cloning from the original Lyme-disease tick isolate obtained by A. Barbour (Johnson, et al., 1984). It should be noted that other microbial DbpA’s, such as E. coli (ATP-dependent RNA helicase DbpA), are significantly different to Borrelia DbpA. B. burgdorferi sensu stricto has a linear plasmid (lp54) which carries a two-gene operon encoding two surface lipoproteins, DbpA and DbpB, both of which bind decorin (Guo, et al., 1995).
DbpA and DbpB are surface-exposed outer membrane lipoproteins that mediate the attachment of Borrelia to decorin, a major component of the host extracellular matrix, enabling bacteria to colonize mammalian tissues (Roberts, et al., 1998). Both can mediate interaction with the glycosaminoglycans (GAGs) heparin and dermatan sulfate (Guo, et al., 1998), but only DbpB binds chondroitin sulfate (Fischer, et al., 2003). The expression of decorin-binding protein (Dbp) A and/or DbpB is sufficient to convert a high-passage nonadherent B. burgdorferi strain into one that efficiently binds HEK293 epithelial cells.
The spirochete travels from the tick mid-gut during tick feeding, to the tick salivary glands and into the mammal host, and it is believed that this migration is facilitated by changes in expression of different B. burgdorferi genes. It is thought that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. While not expressed in the unfed tick, DbpA (and possibly DbpB) are quickly upregulated, either during feeding or after entry, into the host. The location of DbpA and DbpB in the outer membrane of B. burgdorferi allows exposure of these proteins to the host immune system. DbpA has also been used to show high levels of genetic diversity in B. afzelii-infected rodent hosts, by qPCR (Coipan, et al., 2018).
NMR and a crystal structure of a DbpA monomer (resolution of 1.60 Å) confirmed three lysines co-localize with decorin to a common basic patch near the C terminus of the protein (Wang & Feng, 2015; Fortune, et al., 2014). Knockout strains confirmed that the lysine residues are required for binding and infection in mice (Fortune, et al., 2014). Strain-specific variations of Borrelia surface proteins also affect tissue tropism (Lin, et al., 2014) as well as differences in GAG binding affinities which is correlated with differences in GAG-binding pocket location and epitope number (Morgan & Wang, 2015).
REFERENCES
- Fischer, J. R., Parveen, N., Magoun, L. & Leong, J. M., 2003. Decorin-binding proteins A and B confer distinct mammalian cell type-specific attachment by Borrelia burgdorferi, the Lyme disease spirochete. Proc. Natl. Acad. Sci. U. S. A. , Volume 100, p. 7307–7312.
- Casselli, T., Tourand, Y. & Bankhead, T., 2012. Altered murine tissue colonization by Borrelia burgdorferi following targeted deletion of linear plasmid 17-carried genes. Infection and Immunity, 80(5), pp. 1773-1782.
- Coipan, C. E. et al., 2018. The genetic diversity of Borrelia afzelii is not maintained by the diversity of the rodent hosts. Parasit Vectors, 11(1), p. 454.
- Fortune, D. E. et al., 2014. Identification of Lysine Residues in the Borrelia burgdorferi DbpA Adhesin Required for Murine Infection. Infect Immun., 82(8), p. 3186–3198.
- Guo, B. P. et al., 1998. Decorin-binding adhesins from Borrelia burgdorferi. Mol. Microbiol. , Volume 30, p. 711–723.
- Guo, B. P., Norris SJ, S. J., Rosenberg, L. C. & Hook, M., 1995. Adherence of Borrelia burgdorferi to the proteoglycan decorin. Infect. Immun., Volume 63, p. 3467–3472.
- Johnson, R.C., et al. 1984. Borrelia burgdorferi sp. nov.: etiologic agent of Lyme disease. Int J Syst Bacteriol, 34, pp. 496–497.
- Lin, Y. P., Benoit, V., Yang, X. & Martínez-Herranz, R., 2014. Strain-specific variation of the decorin-binding adhesin DbpA influences the tissue tropism of the lyme disease spirochete. PLoS Pathog. , 10(7), p. 14.
- Morgan, A. M. & Wang, X., 2015. Structural mechanisms underlying sequence-dependent variations in GAG affinities of decorin binding protein A, a Borrelia burgdorferi adhesin. Biochem J., 467(3), pp. 439-51.
- Roberts, W. C., Mullikin, B. A., Lathigra, R. & Hanson, M. S., 1998. Molecular analysis of sequence heterogeneity among genes encoding decorin binding proteins A and B of Borrelia burgdorferi sensu lato. Infect. Immun., Volume 66, p. 5275–5285.
- Wang, X. & Feng, W., 2015. Structure of decorin binding protein B from Borrelia burgdorferi and its interactions with glycosaminoglycans. Biochim Biophys Acta., 1854(12), pp. 1823-1832.
This Borrelia burgdorferi DbpB recombinant protein has been manufactured to aid the development of in vitro diagnostics.
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培养皿上的细菌是一个月前接种的,培养基成分没有变化
水浴恒温摇床可分为常温水浴恒温摇床(室温~100℃)和冷冻水浴恒温摇床(常用为0~100℃,也可定制更低温度的如:-10℃~100℃)。在运行方式上可分为往复式、回旋式和双功能水浴恒温摇床。气浴恒温摇床也可分为常温恒温摇床和冷冻恒温摇床。常温气浴恒温摇床的温度范围为:室温+5~60℃。冷冻气浴恒温摇床的温度范围为:4~60℃。在运行方式上也分为往复式、回旋式和双功能气浴恒温摇床。你可以根据实验的具体要求来选择合适的摇床。向左转|向右转向左转|向右转向左转|向右转
2.溶氧,在好氧培养过程中,空气是滤过开放的,所以通过摇到可以让更多空气中氧气溶解于发酵液中。厌氧则不是这个作用了。 3.体系均一,有便于对不同参数的取样测定。。
2,适宜的床面横向坡度。
3,冲洗水大小要适宜。
4,给矿量适当切均匀。
5,给矿浓度适宜。
6,物料在入选前的准备。
7,物料在床面的分带和产品的截取。
操作的时候还有一些细节,有什么问题可以咨询河南红星机器,摇床生产出口基地,30年的生产经验,可以免费安装调试维修。
光生化研究用的就有:脱色摇床,反应摇床,培养摇床;
培养摇床又分水浴摇床和气浴摇床(又称恒温振荡培养箱)。可见恒温培养箱与摇床不是一个概念,只是两者有交集————恒温振荡培养箱既属摇床的一个分支,又属恒温培养箱的一个分支。
暂无品牌问答