Highlights
- Simple, rapid recovery of ultra-pure DNA from PCR, endonuclease digestions, and cell-free DNA preps, etc.
- Unique column construction allows sample loading and washing to be performed using a centrifuge, microcentrifuge, or vacuum source.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
Description
Detergent Tolerance | ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
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Elution Volume | ≥ 2 ml |
Equipment | Microcentrifuge and centrifuge or vacuum source. |
Purity | Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
Sample Source | DNA from large-scale sample sources including restriction endonuclease digestions and "crude" DNA preparations from cell-free lysates. |
Size Range | 50 bp to 23 kb |
Yield | ≤ 500 µg total DNA can be recovered.For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
Q1: What is the minimum input volume of DNA sample?
Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.
Q2: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
Q3: How many times can columns be reloaded?
We recommend no more than 5 times as binding efficiency might decrease.
Q4: How to process naked DNA stored in DNA/RNA Shield?
Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size.
Q5: What is the lower limit and minimal amount of DNA that can be recovered?
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Q6: What to do if ethanol addition to the DNA Wash Buffer was omitted?
The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
“I sampled this kit next to a kit we already use. I had three of the same sample, two for the Zymo kit and one for the other. On one of the ones designated for your kit, I accidentally added the sample directly to the column filter, then added other reagents (I figured I might as well try it). The other I did as per the instructions. The one on which I goofed gave a similar purification and concentration as the other kit, while the one I did properly yielded approximately four times the DNA per µL as the other kit. I am very happy with this result, and when I rerun my samples, I plan to use your product.”
-Rosalind P. (University of Arkansas for Medical Sciences)
“Almost no loss of elution buffer at the final step. It is quite impressive compared to the other kits I have used, which lose about 5-7µl.”
-Joseph R. (Miller School of Medicine, University of Miami)
“It was a very simple procedure and it gave a concentration ten times the original amount.”
-Kimberly M. (University of Pennsylvania)
Read MoreCat # | Name | Size | Price | |
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D3004-4-16 | DNA Elution Buffer | 16 ml | $18.00 | |
D3004-4-50 | DNA Elution Buffer | 50 ml | $32.00 | |
D4003-1-L | DNA Binding Buffer | 50 ml | $33.00 | |
D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
D4004-1-L | DNA Binding Buffer | 100 ml | $57.00 | |
D4003-2-48 | DNA Wash Buffer (Concentrate) | 48 ml | $60.00 | |
C1013-10 | Zymo-Spin VI Columns | 10 Pack | $44.00 | |
C1013-20 | Zymo-Spin VI Columns | 20 Pack | $68.00 |
ebiomall.com
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超净工作台(cleanbench)是为了适应现代化工业、光电产业、生物制药以及科研试验等领域对局部工作区域洁净度的需求而设计的。超净工作台原理是在特定的空间内,室内空气经预过滤器初滤,由小型离心风机压入静压箱,再经空气高效过滤器二级过滤,从空气高效过滤器出风面吹出的洁净气流具有一定的和均匀的断面风速,可以排除工作区原来的空气,将尘埃颗粒和生物颗粒带走,以形成无菌的高洁净的工作环境。
与超净工作台不同,生物安全柜是为操作原代培养物、菌毒株以及诊断性标本等具有感染性的实验材料时,用来保护操作者本人、实验室环境以及实验材料,使其避免暴露于上述操作过程中可能产生的感染性气溶胶和溅出物而设计的。超净工作台只能保护在工作台内操作的试剂等不受污染,并不保护工作人员,而生物安全柜是负压系统,能有效保护工作人员。目前实验室中,超净工作台基本能满足大部分实验操作需要。
超净工作台的使用
1使用前检查
(1)接通超净工作台的电源。
(2)旋开风机开关,使风机开始正常运转,这时应检查高效过滤器出风面是否有风送出。
(3)检查照明及紫外设备能否正常运行,如不能正常运行则通知工程部检验。
(4)工作前必须对工作台四周环境及空气进行超净处理,认真进行清洁工作,并采用紫外线灭菌法进行灭菌处理。
(5)净化工作区内严禁存放不必要的物品,以保持洁净气流活动不受干扰。
2使用方法
(1)使用工作台时,先经过清洁液浸泡的纱布擦拭台面,然后用消毒剂擦拭消毒。
(2)接通电源,提前50分钟打开紫外灯照射消毒,处理净化工作区内工作台表面积累的微生物,30分钟后,封闭紫外灯,开启送风机。
(3)工作台面上,不要存放不必要的物品,以保持工作区内的洁净气流不受干扰。
(4)操纵结束后,清理工作台面,收集各废弃物,封闭风机及照明开关,用清洁剂及消毒剂擦拭消毒。
(5)最后开启工作台紫外灯,照射消毒30分钟后,封闭紫外灯,切断电源。
(6)每仲春用风速计丈量一次工作区均匀风速,如发现不符合技术标准,应调节调压器手柄,改变风机输进电压,使工作台处于最佳状况。
(7)每月进行一次维护检查,并填写维护记录。
3清洁:
(1)每次使用完毕,立即清洁仪器,悬挂标识,并填写仪器使用记录。
(2)取样结束后,先用毛刷刷往洁净工作区的杂物和浮尘。
(3)用细软布擦拭工作台表面污迹、污垢目测无清洁剂残留,用清洁布擦干。
(4)要经常用纱布沾上酒精将紫外线杀菌灯表面擦干净,保持表面清洁,否则会影响杀菌能力。
(5)效果评价:设备内外表面应该光亮整洁,没有污迹。
4.超净工作台注意事项
(1)每次使用前开机5分钟让设备自净;
(2)注意阅读超净工作台的使用说明书
(3)定期更换超净工作台的初效过滤器和高效空气过滤器
文章来源:英格恩技术的官方博客,原文详见:http://www.engreen.cn/超净工作台的使用
而且,紫外在培养基中造成的富氧离子会杀伤细胞。
望采纳^O^
测流式由侧面通过工作台(左右 上下皆可),于是缺点就是在气流层边缘可能出现负压,导致污染。而且其较为封闭,只有手伸入的圆洞,取拿物品较为
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