The Bcl-2 gene family encodes a set of proteins involved in apoptotic inhibition, which are commonly analyzed during the performance of a Bcl-2 apoptosis assay. The ApoPrimer Set (Bcl-2 family) for RT-PCRis composed of a set of primers used toamplify cDNA derived from the mRNA of seven Bcl-2 family members (mcl-1, bfl-1, bax-alpha, bcl-2, bak, bik, bcl-x), as well as a beta-actin control. A positive control RNA is also included. The beta-actin primer is a useful internal control for sample quantification and for comparison between samples. PCR products generated from the positive control RNA can be distinguished from target mRNA due to size differences of the amplified products.
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2、仪器功能:进口和国产仪器主要功能:如保存,过温保护,断电保护,热盖功能等基本上是一样的,只是附带功能上有所差别,比如:进口的显示屏是大型的触摸式荧屏操作,国产的大多是触摸键,荧屏显示操作,进口仪器有的可以升级为荧光定量PCR仪,而国产的目前主要是专用机。而这些仪器附带功能的差别大多都是最近两年新推出来的,价格确是相差几倍。有的进口仪器没有这些附带的特殊功能,从性能和功能上比有的等于或低于国产的。
请问PCR仪设置加样量有什么作用,我们实验室有的pcr仪可以设置有的不能设置。如果我用了25微升体系,pcr仪设置时设置了50微升体系会怎么样呢?帮忙回答一下。谢谢~
实验室很早就买的这个PCR仪,一直放着没有用,现在想用了,找不到说明啦,求帮助
做完实验之后放8联排的卡槽怎么按都弹不出来了。求助各位可能的原因有哪些?原因越详细越多越好,避免下次再出现这种状况,有没有其他战友遇到过这种情况?谢谢!
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