Description:
UsetheCFXConnectReal-TimePCRDetectionSystemfortwo-targetanalysis.The96-wellreactionmoduleoffersexcellentthermalperformanceanduniformityacrosstheentireblock.Multipledataacquisitionmodes,includingafast-scanoption,providetheflexibilitytomeetallyourreal-timePCRneeds.
FeaturesandBenefits
- Designedforcontrolbyremotepersonalcomputer(upto4CFXSystems)
- Detectionofupto2targetsperwell,plusachanneldedicatedtosingleplexFRET
- IntegratedsolutionforyourSYBR®GreenorEvaGreenandduplexexperiments
- ThermalgrADIentfeatureidentifiesoptimalannealingtemperatureinasinglerun
TheCFXConnectReal-TimePCRDetectionSystemfeaturessixindependentlycontrolledthermalelectricunits,providingeven,preciselycontrolledtemperaturesatalltimesduringtherun,includingramping.Thereducedmassofthehoneycombblockprovidesfastrampingandreducedsettlingtime(timetoachievethermaluniformity).Theprogrammablethermalgradientfeatureallowsatemperaturegradientofupto24°Cacrossthereactionblockatanystepinaprotocol.
Theopticalsystem,withthreefilteredLEDsandthreefilteredphotodiodes,collectsdatafromallwells,detectinguptotwotargetsperwell.Forsingle-colorFAM,EvaGreen,andSYBRGreenIAssays,thefast-scanoptionreadssingle-channelfluorescenceinall96wellsinjust3seconds.AseparatechannelwithanLEDfilter–photodiodecombinationisdedicatedtoFRETsingleplexexperiments.
ProductComponents
- CFXConnectThermalCyclerchassis
- CFXConnect96-WellOpticalReactionModule
- USBcable
- Instructionmanual
ConfigurationOptions
TheCFXConnectReal-TimePCRDetectionSystemisavailablewithastarterpackagewithSYBRGreenSupermix,96-wellPCRplates,andplateseals(1855200).
TheCFXConnectReal-timePCRDetectionSystemcanbeconfiguredwithotherPCRinstruments,sothatitcanbesoftware-controlled.InstrumentpermutationsallowuptofourdifferentCFXConnect,CFX96™,orCFX384™SystemstobecontrolledbyonePC.Youcanstartthesameexperimentonallfourinstrumentsatonce,orstartarunononeinstrumentwhileanotherisrunningadifferentprogram.
CombineanytwoCFXReal-TimePCRDetectionSystemswiththeCFXAutomationSystemII,ourroboticplatehandler,tocreateahigh-throughput,automatedreal-timePCRsystemwithacompactfootprint.
ServicePlansandValidationToolsforCFXSystems
- Ensurethatyourreal-timePCRsystemsarefunctioningoptimallywithBio-RadExpertCareService;choosefromseveralserviceplanstokeepyourCFXAutomationSystemIIrunningitsbest
- CertifysysteminstallationandvalidatesystemperformancewithCFXSystemIQ/OQServicesandCFXAutomationSystemIIIQ/OQServices
- VerifytheperformanceofyourCFXSystemswithThermalValidationServicesandtheCFXQualificationPlates
RelatedProducts
- PX1™PCRPlateSealer
- PCRplasticsoptimizedforCFXReal-TimePCRDetectionSystems:
- Hard-Shell®96-WellLowProfileSkirtedPCRPlatesincludingbarcodedplates(HSP9955)
- Hard-Shell384-WellSkirtedPCRPlatesincludingbarcodedplates(HSP3905)
- Microseal®"B"AdhesiveSeals(MSB1001)
- OpticallyClearHeatSeals(1814030)
- CFXMaestroSoftware,SecurityEdition,forcompliancewithU.S.FDA21CFRPart11regulations
- CFXQualificationPlateforperformanceverification,96-well (1845098)
- CFXQualificationPlateforperformanceverification,384-well (1845099)
RelatedCategories
- Real-TimePCRDetectionSystems
- PCRReagentsandqPCRReagents
- PCRPlasticConsumables
- PCRSeals
- PCRAnalysisSoftware
SupportingDocuments
- CFXConnectReal-TimePCRDetectionSystemSpecificationsSheet
- ConnectReal-TimePCRSystemInstallationQuickGuide
- CFXConnectReal-TimePCRDetectionSystemInstrumentGuideInstructionManual
- Real-TimePCRApplicationsGuide
OnlineResources
- Real-TimePCRDetectionSystemsSelectionChart
- qPCR/Real-TimePCR
- qPCRInstrumentation
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2、仪器功能：进口和国产仪器主要功能：如保存，过温保护，断电保护，热盖功能等基本上是一样的，只是附带功能上有所差别，比如：进口的显示屏是大型的触摸式荧屏操作，国产的大多是触摸键，荧屏显示操作，进口仪器有的可以升级为荧光定量PCR仪，而国产的目前主要是专用机。而这些仪器附带功能的差别大多都是最近两年新推出来的，价格确是相差几倍。有的进口仪器没有这些附带的特殊功能，从性能和功能上比有的等于或低于国产的。
请问PCR仪设置加样量有什么作用，我们实验室有的pcr仪可以设置有的不能设置。如果我用了25微升体系，pcr仪设置时设置了50微升体系会怎么样呢？帮忙回答一下。谢谢~
实验室很早就买的这个PCR仪，一直放着没有用，现在想用了，找不到说明啦，求帮助
做完实验之后放8联排的卡槽怎么按都弹不出来了。求助各位可能的原因有哪些？原因越详细越多越好，避免下次再出现这种状况，有没有其他战友遇到过这种情况？谢谢！
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