AQUApure Tetra-Ub Chains (K33-linked) Protein, CF Summary
Ub-AQUA analysis:
K33: 95.08%
K48: 2.69%
K63: 0.90%
K11: 0.71%
All other linkages ≤ 0.38%
Ubiquitin chains vary in length, linkage, and function. K33-linked Tetra-Ubiquitin Chains (Ub4) are ideal for investigating Ubiquitin-binding proteins and as substrates for Ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. IMPORTANT: Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40 °c="" for="" 20="" minutes="" prior="" to="" gel="" electrophoresis.="">
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
UC-103
| Formulation | 1 mg/ml (29 μM) in sterile, deionized water. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Tetra-Ubiquitin
Linkage specific Poly-Ubiquitin chains may be used as a substrate for in vitro reactions with deubiquitinating enzymes ("DUB"s") that cleave the peptide or isopeptide linkage between adjacent Ubiquitin molecules. Poly-Ubiquitin chains can also be used to investigate mechanisms of binding and recognition between the chains and other proteins that contain Ubiquitin-Associated domains (UBAs), Ubiquitin-interacting motifs (UIMs), ZnF"s and/or other Ubiquitin-sensing elements.
K33-linked Tetra-Ubiquitin chains are manufactured using recombinant Ubiquitin and purely enzymatic techniques to avoid the potential for contaminating synthetic intermediates. The correctness of linkage and purity of each production lot is assessed using the Absolute Quantitation of Ubiquitin method (Ub-AQUA), an LCMS-based technique that provides extremely accurate information on the composition of Poly-Ubiquitin samples.
- Kirkpatrick D.S., et al. (2006) Nat Cell Biol. 8(7): 700-10
- Michel, M.A. et al. (2015) Mol Cell. 58(1): 95-109
- Ordureau, A., et al. (2014) Mol. Cell 56(3): 360–375
- Ordureau, A., et al. (2015) Pro. Nat. Acad. of Sci. USA 112(21): 6637–6642
- Phu L., et al. (2011) Mol Cell Proteomics 10(5): M110.003756
Citations for AQUApure Tetra-Ub Chains (K33-linked) Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
3Citations: Showing 1 - 3Filter your results:
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- ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome StabilityAuthors: P Haahr, N Borgermann, X Guo, D Typas, D Achuthanku, S Hoffmann, R Shearer, TK Sixma, N MailandMol. Cell, 2018;0(0):.Species: HumanSample Types: Cell LysatesApplications: Bioassay
- USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilizationAuthors: S Yang, L Liu, C Cao, N Song, Y Wang, S Ma, Q Zhang, N Yu, X Ding, F Yang, S Tian, K Zhang, T Sun, J Yang, Z Yao, S Wu, L ShiNat Commun, 2018;9(1):1285.Applications: Bioassay
- Novel polyubiquitin imaging system, PolyUb-FC, reveals that K33-linked polyubiquitin is recruited by SQSTM1/p62Authors: Y Nibe, S Oshima, M Kobayashi, C Maeyashiki, Y Matsuzawa, K Otsubo, H Matsuda, E Aonuma, Y Nemoto, T Nagaishi, R Okamoto, K Tsuchiya, T Nakamura, S Nakada, M WatanabeAutophagy, 2017;0(0):1-43.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
FAQs
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以前先放-80再液氮，但是去-80冰箱有点远，我想能不能直接放液氮里面，我直接放过复苏成活率低了很多，有没有这种冻存盒可以直接放液氮的？
要做细胞缺氧，需要买细胞缺氧盒，但不知道哪里有卖，各位大神有没有曾经做过的，能不能告诉我在哪能购买到，哪个公司有卖。。急。。。。。。实验小白。。。。在下先在此谢过了。
心肌肌钙蛋白I，英文为CardiactroponinI，该试剂盒在POCT领域内几乎是每个厂家都必做的项目，也是多数医院科室都开展的收费项目，市场容量很大，全国一年下来保守估计有3千万人份。但有些厂家销售的特别好，有些厂家销售的很一般。这一是由于销售渠道所致、二是因为产品质量差导致。试剂盒质量差一是因为厂家自身调试工艺不太好，二是由于所选择的抗体质量不好所致。现在市面上有心肌肌钙蛋白I抗体的厂家不多，大概也就不超过10家吧，主要为进口品牌，如：HyTest、BBI、Biospacific、Medix，国产品牌为深圳菲鹏，杭州启泰。其中着重要说的是杭州启泰，这是一家成立7年的抗原、抗体研发公司，公司不大，但立足于自主研发、创新，最近新推出的cTnI抗体可以说是目前国内外最好的抗体，这也算是填补了国内该领域的空白。从事了该行业将近10年，发此贴也是真切希望国内的生物试剂类公司，都专注于研发、创新，做一些好的试剂出来。因为很多事情国内做的并不比国外差的，国内的生物同行请加油吧!
小弟现有一事儿想求助！
如下：1）凋亡的时候细胞色素c由线粒体里释放到胞浆里去的。那么如果能够测到此时在胞浆里的细胞色素c表达增多的话应该就能说明问题了
（2）可此时的问题是如果提胞浆蛋白时把线粒体也裂解了话细胞色素c也会被释放出来，还是会在胞浆里大量表达！就说不清楚是凋亡由线粒体释放的还是被人为的破坏了线粒体导致了的！！！
（3）故我的问题就是该怎么样去除这个人为的影响？怎么说明我测的胞浆里的细胞色素c就是凋亡时线粒体释放的？换句话讲就是如果能够在用细胞裂解液裂解胞浆蛋白时，将线粒体剔除掉？
是不是买个细胞色素c的测试盒就可以了啊？这个盒子有人用过吗，请指教一二！
盼复！非常感谢！
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