- glucagon receptor antagonists 1
- glucagon receptor antagonists 2
- glucagon receptor antagonists 3
- MK 0893
Exendin-4GLP-1 activator |
Sample solution is provided at 25 µL, 10mM.
Quality Control & MSDS
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- Purity = 98.79%
- COA (Certificate Of Analysis)
- HPLC
- MS (Mass Spectrometry)
- MSDS (Material Safety Data Sheet)
- Datasheet
Chemical structure
Cell experiment [1]: | |
Cell lines | Mouse insulinoma beta TC-1 cell line |
Preparation method | Soluble to 1 mg/ml in sterile water. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months. |
Reacting condition | 0.1 nM to 1 μM for 2 h |
Applications | Exendin-4, like GLP-1, could stimulate dose dependently the glucose-induced insulin secretion in isolated rat islets, and, in mouse insulinoma beta TC-1 cells, both peptides were able to stimulate the proinsulin gene expression at the level of transcription. |
Animal experiment [2]: | |
Animal models | Ob/ob mice model |
Dosage form | 10 μg/kg or 20 μg/kg, qd for 14 days |
Application | Ob/ob mice were treated with Exendin-4 [10 μg/kg or 20 μg/kg] for 60 days. It was found that Ob/ob mice sustained a reduction in the net weight gained during Exendin-4 treatment. Serum glucose and hepatic steatosis was significantly reduced in Exendin-4 treated ob/ob mice. Moreover, Exendin-4 improved insulin sensitivity in ob/ob mice, as calculated by the homeostasis model assessment. The measurement of thiobarbituric reactive substances as a marker of oxidative stress was significantly reduced in ob/ob-treated mice with Exendin-4. |
Other notes | Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1] Gke R, Fehmann H C, Linn T, et al.Exendin-4 is a high potency agonist and truncated exendin-(9-39)-amide an antagonist at the glucagon-like peptide 1-(7-36)-amide receptor of insulin-secreting beta-cells[J]. Journal of Biological Chemistry, 1993, 268(26): 19650-19655. [2] Ding X, Saxena N K, Lin S, et al.Exendin‐4, a glucagon‐like protein‐1 (GLP‐1) receptor agonist, reverses hepatic steatosis in ob/ob mice[J]. Hepatology, 2006, 43(1): 173-181. |
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Cas No. | 141758-74-9 | SDF | Download SDF |
Synonyms | Exenatide | ||
Chemical Name | N/A | ||
Canonical SMILES | CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)NC(CC1=CNC2=CC=CC=C21)C(=O)NC(CC(C)C)C(=O)NC(CCCCN)C(=O)NC(CC(=O)N)C(=O)NCC(=O)NCC(=O)N3CCCC3C(=O)NC(CO)C(=O)NC(CO)C(=O)NCC(=O)NC(C)C(=O)N4CCCC4C(=O)N5CCCC5C(=O)N6CCCC6C(=O)NC(CO)C(=O)N)NC(=O)C(CC7=CC=CC=C7)NC(=O)C(CC(C)C)NC( | ||
Formula | C184H282N50O60S | M.Wt | 4186.57 |
Solubility | ≥145 mg/mL in DMSO, <2.44 mg/ml="" in="" etoh,="" ≥52="" mg/ml="" in="" h2o="" with="" gentle="" warming="">2.44> | Storage | Store at -20°C |
Physical Appearance | A solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. |
Exendin-4, a glucagon-like protein-1 (GLP-1) receptor agonist, mimics the activity of mammalian incretin hormone glucagon-like peptide 1 (GLP-1), and thus, promotes insulin secretion and functions in the control of glucose [1].
In vitro: Exendin-4 showed a pronounced effect on intracellular cAMP generation. Treatment of GLP-1 in combination with exendin-4 showed additive action on the generation of cAMP. In isolated rat islets and in mouse insulinoma beta TC-1 cells, Exendin-4 stimulated the glucose-induced insulin secretion in a dose dependent manner [2]. In basal forebrain cholinergic neurons, exendin-4 greatly reduced ibotenic acid-induced depletion of choline acetyltransferase immunoreactivity [3].
In vivo: In ob/ob mice, administration of Exendin-4 (10 μg/kg or 20 μg/kg) improved insulin sensitivity and significantly reduced serum glucose and hepatic steatosis. Exendin-4 appeared to effectively reverse hepatic steatosis in ob/ob mice by improving insulin sensitivity [1]. In athymic mice, 63% of exendin-4-treated mice achieved graft function compared with 21% of untreated mice (p = 0.033) in the short-term study. 88% of treated mice had functioning grafts compared with 22% of controls (p = 0.015) in the long-term study. Exendin-4-treated mice gained significantly more weight than the untreated counterparts [4].
References:Ding X, Saxena N K, Lin S, et al. Exendin‐4, a glucagon‐like protein‐1 (GLP‐1) receptor agonist, reverses hepatic steatosis in ob/ob mice[J]. Hepatology, 2006, 43(1): 173-181.Gke R, Fehmann H C, Linn T, et al. Exendin-4 is a high potency agonist and truncated exendin-(9-39)-amide an antagonist at the glucagon-like peptide 1-(7-36)-amide receptor of insulin-secreting beta-cells[J]. Journal of Biological Chemistry, 1993, 268(26): 19650-19655.Perry T A, Haughey N J, Mattson M P, et al. Protection and reversal of excitotoxic neuronal damage by glucagon-like peptide-1 and exendin-4[J]. Journal of Pharmacology and Experimental Therapeutics, 2002, 302(3): 881-888.Sharma A, Srenby A, Wernerson A, et al. Exendin-4 treatment improves metabolic control after rat islet transplantation to athymic mice with streptozotocin-induced diabetes[J]. Diabetologia, 2006, 49(6): 1247-1253.
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心肌肌钙蛋白I,英文为CardiactroponinI,该试剂盒在POCT领域内几乎是每个厂家都必做的项目,也是多数医院科室都开展的收费项目,市场容量很大,全国一年下来保守估计有3千万人份。但有些厂家销售的特别好,有些厂家销售的很一般。这一是由于销售渠道所致、二是因为产品质量差导致。试剂盒质量差一是因为厂家自身调试工艺不太好,二是由于所选择的抗体质量不好所致。现在市面上有心肌肌钙蛋白I抗体的厂家不多,大概也就不超过10家吧,主要为进口品牌,如:HyTest、BBI、Biospacific、Medix,国产品牌为深圳菲鹏,杭州启泰。其中着重要说的是杭州启泰,这是一家成立7年的抗原、抗体研发公司,公司不大,但立足于自主研发、创新,最近新推出的cTnI抗体可以说是目前国内外最好的抗体,这也算是填补了国内该领域的空白。从事了该行业将近10年,发此贴也是真切希望国内的生物试剂类公司,都专注于研发、创新,做一些好的试剂出来。因为很多事情国内做的并不比国外差的,国内的生物同行请加油吧!
以前先放-80再液氮,但是去-80冰箱有点远,我想能不能直接放液氮里面,我直接放过复苏成活率低了很多,有没有这种冻存盒可以直接放液氮的?
要做细胞缺氧,需要买细胞缺氧盒,但不知道哪里有卖,各位大神有没有曾经做过的,能不能告诉我在哪能购买到,哪个公司有卖。。急。。。。。。实验小白。。。。在下先在此谢过了。
小弟现有一事儿想求助!
如下:1)凋亡的时候细胞色素c由线粒体里释放到胞浆里去的。那么如果能够测到此时在胞浆里的细胞色素c表达增多的话应该就能说明问题了
(2)可此时的问题是如果提胞浆蛋白时把线粒体也裂解了话细胞色素c也会被释放出来,还是会在胞浆里大量表达!就说不清楚是凋亡由线粒体释放的还是被人为的破坏了线粒体导致了的!!!
(3)故我的问题就是该怎么样去除这个人为的影响?怎么说明我测的胞浆里的细胞色素c就是凋亡时线粒体释放的?换句话讲就是如果能够在用细胞裂解液裂解胞浆蛋白时,将线粒体剔除掉?
是不是买个细胞色素c的测试盒就可以了啊?这个盒子有人用过吗,请指教一二!
盼复!非常感谢!
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