Description:
KAPADual-IndexedAdapterKit,(15µM)KAPAHyperPrepKits
Shiftyourworkflowintohyperdrive.
TheKAPAHyperPrepKitisaversatile,streamlinedsolutionforDNAlibrarypreparationforIllumina®sequencing.
Thenovelone-tubechemistry,containoptimallyformulatedandevolvedenzymesthatenablehigheryieldsofadapter-ligatedlibraryandloweramplificationbias.Thistranslatestohigherlibrarydiversity,lowerduplicationratesandmoreuniformcoverage,particularlyforFFPEandlow-inputsamples.
NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.
DownloadourAdapterandBeadCalculator
ForResearchUseOnly.Notforuseindiagnosticprocedures.
ProductHighlights
Improvelibraryyieldsandsequencequality
- Higherlibraryyieldstranslatetogreatermolecularcomplexity
- FewercyclesofamplificationwithKAPAHiFiDNAPolymeraseresultsinlowerduplicationratesandimprovedcoverage
Createhigh-qualitylibrariesfromFFPEsamples
- Generatehigh-qualitylibrariesfrom250ngFFPEDNAorless*
- Significantlylowerduplicationratesandhighercoverage*
Completelibraryconstructioninlessthan3hours
- One-tubeworkflowwithminimalcleanupstepsreducesoveralltime,andminimizeshands-ontime
- Sample-to-libraryin<2hoursforPCR-freeworkflows,or<3hourswithamplification*
- Fewerhandlingstepsleadtoimprovedconsistencyandreproducibility
Improveperformancewithlow-inputsamples
- Generatemoresequence-qualitylibrarymoleculeswithoutincreasingadapter-dimers
- Increaseyieldswithoutinducingsizebias
*Dataonfile.
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:
Applications:
Applications- WholeGenomeSequencing
- WholeExomeSequencing
- TargetedSequencing(custompanels)
- ChIP-seq
- AmpliconSequencing
- Methyl-seq
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageKitscanbestoredforupto12months at-20˚C.
Kitscontain KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix
Components
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
各位战友好,因实验要求需要提取人的唾液DNA,但因标本需要在临床采集,无法做到当天就提取DNA,因此天根等漱口水提DNA的试剂盒都不满足实验需求。
之前一直在用艾德莱的唾液DNA保存运输提取试剂盒,但是这段时间下来感觉提取效率太低,而且特别不稳定,经常50%以上的标本都提不出来DNA,或者浓度特别低,完全崩溃的感觉!所以想更换试剂盒或者直接提供唾液DNA的保存液。另国外的这种试剂盒太贵了,因经费问题无法承受,所以希望战友们能够推荐一家效率高些的试剂盒,小女子不胜感激!!
有试剂A和B,传说10-30分钟就可以提取出来。不知道属不属实啊?
而且1000次580块钱!如果有用过的,可否跟我传授一下心得啊?
暂无品牌问答