- Hito Hematoxylin Solution - Single Strength - Recommended for cytological staining.
- Hito Hematoxylin Solution - Double Strength - Recommended for counterstaining of immunohistochemistry and rou
- Description
Details
H&E staining orhematoxylin & eosin staining, is a popularstainingmethod inhistology. It is the most widely used stain in medical diagnosis and research.Hito Hematoxylin Solution is designed on the principle of the Gill’s Hematoxylin method and in three ready-to-use formulation.
- Hito Hematoxylin Solution - Single Strength -Recommended for cytological staining.
- Hito Hematoxylin Solution - Double Strength -Recommended for counterstaining of immunohistochemistry and routine Histology.
- Hito Hematoxylin Solution - Triple Strength -Recommended for histological staining of nuclei with shorter staining times.
Eosin Y is the most commonly used cytoplasmic stain, but Alcoholic Eosin Solutions are HIGHLY FLAMMABLE and HARMFUL, Hito Eosin-Y Aqueous Solution is a water base solution for fast and safe usage. Hito HE-Diff™Solution is water based and used for Hematoxylin staining differentiation.The tap water in the traditional method is replaced by Hito modified Scott"s Tap Water Substitut, which enables bluing up in a much shorter time and avoids tissue sections falling off from the slides.
250 mlHito HE-Diff™ Solution Manual and MSDS
- Additional Information
Additional Information
SKU HTSHS0113 - Reviews
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平常都是用超声破清洗器水浴超声破碎大约20分钟,然后上样的
但是一方面,常温超声不知道会不会造成蛋白降解破坏,因为发现加冰以后超声会变得非常弱,所以都么加冰。另一方面,因为水浴超声声强是不均匀的,如果样品多的话,会发现有些已经超碎DNA,但有些还是一团。
不知道是否有其他更好的方法?
1、安装电泳槽
将有机玻璃的电泳凝胶床洗净,晾干,用胶带将两端的开口封好,放在水平的工作台上,插上样品梳。
2、琼脂糖凝胶的制备
称取琼脂糖溶解在电泳缓冲液中,(按0.3-1.5%的琼脂糖含量,1-25kb大小的DNA用1%的凝胶,20-100kb的DNA用0.5%的凝胶,200-2000bp的DNA用1.5%的凝胶)置微波炉或沸水浴中加热至完全溶化(不要加热至沸腾),取出摇匀。
3、灌胶
将冷却到60℃的琼脂糖溶液轻轻倒入电泳槽水平板上。
4、待琼脂糖胶凝固后,在电泳槽内加入电泳缓冲液,然后拔出梳子。
5、加样
将DNA样品(DNA样品是细胞破碎之后用离心管离心沉淀获得的)与加样缓冲液按4:1混匀后,用微量移液器将混合液加到样品槽中,每槽加10-20μl,记录样品的点样次序和加样量。
6、电泳
安装好电极导线,点样孔一端接负极,另一端接正极,打开电源,调电压至3-5V/cm,电泳1-3hr,当溴酚蓝移到距凝胶前沿1-2cm时,停止电泳。
7、染色和观察
取出凝胶,放在含有溴化乙锭的染色液中染色30min,即可在254nm的紫外灯下观察,有橙红色荧光条带的位置,即为DNA条带,或在紫外灯下照相记录电泳图谱。溴化乙锭是致癌剂,操作时要小心,必须戴手套。
我怀疑溶菌酶有问题,因为其消化的30min内菌液几乎没大变化,而我曾看到别人用溶菌酶消化时菌液变的挺粘稠。
今下午再做时还是没什么变化,但发现溶菌酶在冰上静置2-3h后竟然沉淀下来了,而且上清和下面的白色溶菌酶分界明显,是我的溶菌酶配的有问题吗?出现这种情况溶菌酶还能用吗?我是-20度保存的。
下一步怎么处理菌液呢?
请高手多多指教!
暂无品牌问答