Recommendedworkingmedium,trypsinvolume andcellinoculationdensity (7/9/99) TypeofVessels CultureArea(cm2) Volume(ml) WorkingMedium(ml) InoculationDensity VolumeofTrypsin(ml) NunclonFlask 24 50 7 4.8x105 0.5-0.8 CorningFlask 25 70 7 5x105 0.5-0.8 FalconFlask 75 250 25 1.5x106 1.0 NunclonFlask 83 260 30 1.6x106 1.2 NunclonFlask 175 800 68 3.5x106 2.0 FalconDish35x10mm 9.6 2.5 1.6x105 0.2-0.3 FalconDish65x15mm 21 6.0 4.2x105 0.5-0.6 FalconDish100x20mm 58 16.0 1.1x106 1.0 96wellPlate 0.32 0.2 6x103 0.01-0.02 24wellPlate 1.88 1.5 3.7x104 0.08-0.1 12wellPlate 3.83 2.0 7.6x104 0.1-0.2 6wellPlate 9.4 3.0 1.8x105 0.2-0.3Author:Long-ChengLi Source: Abstract:Usefuldataforcellsubculturewithdifferentculturevessels.