细胞生物学专业
来自 : 蚂蚁淘
SECTIONS4micronparaffinwaxsections
SOLUTIONS
1.AlcianBluepH2.5
- AlcianBlue1g
- 3%AceticAcid100ml
2.1%PeriodicAcid3.SchiffsReagent
METHOD
- Takesectionstowater
- PlaceinAlcianBluefor5minutes
- Washindistilledwater
- Placein1%periodicacidfor10minutes
- Rinsewellindistilledwater
- PlaceinSchiffsreagentfor20minutes
- Washinrunningtapwaterfor10minutes
- StainlightlyinGillsHaematoxylin
- Washand"blue"in1%Lithiumcarbonate
- Dehydrate,clear.MountsectionsinDPX
RESULTS- AcidMucins-Blue
- NeutralMucins-Magenta
- Nuclei-PaleBlue
NOTE:NoresponsibilityisassumedbyTheUniversityofNottinghamortheQueensMedicalCentreNHSTrustforanyinjuryand/ordamagetopersonsorpropertyasamatterofproductsliABIlity,negligenceorotherwise,orfromanyuseoroperationofanymethods,products,instructionsorideascontainedinthematerialherein.ItistheusersresponsibiltytoensurethatallproceduresarecarriedoutaccordingtoappropriateHealthandSafetyrequirements.
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