Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Based on immunoelectrophoresis and/or ELISA, the antibody reacts with peroxidase from horserADIsh roots.It may cross-react with peroxidase from other sources.
Physical State: Sterile-filtered liquid
Aliquot and freeze undiluted product at -20°C or below. Avoid repeated freezing and thawing. Prepare working dilution fresh each day.
Expiration date: one year from date of receipt. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Buffer: 0.01M Sodium Borate - Sodium Phosphate, 0.15M NaCl, pH 8.5
StABIlizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative:0.05% Sodium Azide
Suggested Working Concentration or Dilution Range:
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeABIlity, etc. The actual dilution used must be determined empirically.
18 nm Colloidal Gold
Colloidal gold reagents for transmission and scanning electron microscopy (EM Grade) are distinguished from other commercial preparations by careful separation of monomeric particles from small aggregates using ultracentrifugation in density grADIents. The resulting monomeric colloidal gold-protein complexes are sUSPended in sterile-filtered buffer containing stABIlizers and a preservative. All particle sizes in the EM Grade category may also be used for light microscopy and immunoblotting by those who prefer to use particles larger than 4 nm.