Antibioticsshouldbeaddedtolowerthan60Cbroth,orfiltersterilized:Seenote."C"referstochromosomalresistance:"P"plasmidbasedresistance,"FS"filtersterilizationrequired.Temperatureisrequiredforstorage.Concentration(µg/ml)AntibioticsforPseudomonas,E.Coli,Agrobacterium,etc...Ampicillin1000(C/P)50(P)Stock:25mg.mlNasalt:FS:Storeat-20C,carbenicillinismorestablethanAmp.Carbenicillin1000(C/P)50(P)100mg/ml:FS:-20CChlroramphenicol500(C)30(C/P)170µg/mlforplasmidamplification34mg/mlinEtOH:-20CD-cycloserine200(C)AdddryafterautoclavingErythromycin200(C)25mg/mlEtOH:-20CGentamycin10(C/P)AdddryafterautoclavingGeneticin200forfungiSameaskanamycininmodeofactionandpreparationHygromycin250forfungi25mg/ml:FS:-20CKanamycin200(P)50(P)25mg/ml:FS:-20CKasugamycin200(C)20mg/ml:FS:-20CMercuricchloride40(C/P)10µg/mlifinminimalmedia,40mg/mlinEtOH:-20CNalidixicacid1000(C)20(C)100mg/mlinH2O,add6NNaOHuntildissolved:4CNeomycin200(P)50(P)25mg/ml:FS:-20CRifampicin200(C)200mg/mlmakefreshinMethanolSpectinomycin200(C/P)25(P)20mg/ml:FS:-20CStreptomycin200(C/P)25(P)20mg/ml:FS:-20CSulfamethoxazole500(C)AdddryafterautoclavingTriomethoprim500(C)AdddryafterautoclavingTetracycline20(C)50(P)15(P)12.5mg/ml50%EtOH:-20C
